Microfluidic valve with cored glass microneedle for microinjection

Sang Hoon Lee, Wonje Jeong, David J. Beebe

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

In this paper, a new microinjection device was constructed by fusing a glass microneedle and a PDMS-based microvalve. The microneedle was fabricated via traditional micropipette pulling. The PDMS-based microvalve regulates the fluid flow in the microchannel and microneedle. The 'ON/OFF' operation of the valve was controlled by manually supplied pneumatic pressure. The valve membrane utilized a two level geometry to improve control at low flow rates. The relation between pressure and flow was measured and the results showed that very small volumes of fluid (less than 1 nl) could be controlled. The valve operation was investigated by monitoring the tip of the needle and pneumatic pressure simultaneously and it demonstrated very stable 'ON/OFF' operation to the pressure change.

Original languageEnglish
Pages (from-to)164-167
Number of pages4
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume3
Issue number3
DOIs
Publication statusPublished - 2003 Aug 1
Externally publishedYes

Fingerprint

Microfluidics
Microinjections
Glass
Pressure
Pneumatics
Microchannels
Needles
Flow of fluids
Flow rate
Membranes
Equipment and Supplies
Fluids
Geometry
Monitoring

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Microfluidic valve with cored glass microneedle for microinjection. / Lee, Sang Hoon; Jeong, Wonje; Beebe, David J.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 3, No. 3, 01.08.2003, p. 164-167.

Research output: Contribution to journalArticle

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