Microfluidic valve with cored glass microneedle for microinjection

Sanghoon Lee, Wonje Jeong, David J. Beebe

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)


In this paper, a new microinjection device was constructed by fusing a glass microneedle and a PDMS-based microvalve. The microneedle was fabricated via traditional micropipette pulling. The PDMS-based microvalve regulates the fluid flow in the microchannel and microneedle. The 'ON/OFF' operation of the valve was controlled by manually supplied pneumatic pressure. The valve membrane utilized a two level geometry to improve control at low flow rates. The relation between pressure and flow was measured and the results showed that very small volumes of fluid (less than 1 nl) could be controlled. The valve operation was investigated by monitoring the tip of the needle and pneumatic pressure simultaneously and it demonstrated very stable 'ON/OFF' operation to the pressure change.

Original languageEnglish
Pages (from-to)164-167
Number of pages4
JournalLab on a Chip
Issue number3
Publication statusPublished - 2003 Aug

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering


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