Microfluidics-generated pancreatic islet microfibers for enhanced immunoprotection

Yesl Jun, Min Jun Kim, Yong Hwa Hwang, Eun Ae Jeon, Ah Ran Kang, Sang Hoon Lee, Dong Yun Lee

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Pancreatic islet transplantation is a promising method for treatment of type 1 diabetes mellitus. However, transplanted islets can be destroyed due to host immune reactions. To immunologically protect transplanted islets, here an immunoprotective microfiber including islets by using a polydimethylsiloxane (PDMS)-based microfluidic device is newly designed. A cylindrical-flow channel in the microfluidic platform is used for producing collagen-alginate composite (CAC) fibers. This enables mass production and uniform diameter distribution (<250μm) without protruding islets. Collagen, which is the main extracellular matrix component, is added to alginate to mimic the native islet microenvironment. Compared to free islets (control) and alginate-fiber-encapsulated islets, CAC-fiber-encapsulated islets show higher viability and normal insulin secretion. When CAC-fiber-encapsulated islets (1200 islet equivalent) are implanted into the intraperitoneal cavity of streptozotocin-induced diabetic BALB/C mice, the blood glucose levels of all mice return to normoglycemia. Moreover, intraperitoneal glucose tolerance tests demonstrate that islets in the CAC-fiber have similar glucose responsiveness to those of non-diabetic normal mice. These results are attributed to the immunoprotection of the transplanted islets from host immune reactions. On the other hand, all free islets are completely rejected within a week due to severe immune responses. Collectively, fabrication of CAC fibers using microfluidic devices can be used for successful islet transplantation.

Original languageEnglish
Pages (from-to)8122-8130
Number of pages9
JournalBiomaterials
Volume34
Issue number33
DOIs
Publication statusPublished - 2013 Nov 1

Fingerprint

Microfluidics
Alginate
Islets of Langerhans
Collagen
Lab-On-A-Chip Devices
Fibers
Antigen-antibody reactions
Composite materials
Glucose
Islets of Langerhans Transplantation
Inbred BALB C Mouse
Insulin
Polydimethylsiloxane
Channel flow
Glucose Tolerance Test
Streptozocin
Medical problems
alginic acid
Type 1 Diabetes Mellitus
Extracellular Matrix

Keywords

  • Collagen-Alginate composite (CAC)
  • Immunoprotection
  • Islet encapsulation
  • Microfluidics
  • Xenotransplantation

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Jun, Y., Kim, M. J., Hwang, Y. H., Jeon, E. A., Kang, A. R., Lee, S. H., & Lee, D. Y. (2013). Microfluidics-generated pancreatic islet microfibers for enhanced immunoprotection. Biomaterials, 34(33), 8122-8130. https://doi.org/10.1016/j.biomaterials.2013.07.079

Microfluidics-generated pancreatic islet microfibers for enhanced immunoprotection. / Jun, Yesl; Kim, Min Jun; Hwang, Yong Hwa; Jeon, Eun Ae; Kang, Ah Ran; Lee, Sang Hoon; Lee, Dong Yun.

In: Biomaterials, Vol. 34, No. 33, 01.11.2013, p. 8122-8130.

Research output: Contribution to journalArticle

Jun, Yesl ; Kim, Min Jun ; Hwang, Yong Hwa ; Jeon, Eun Ae ; Kang, Ah Ran ; Lee, Sang Hoon ; Lee, Dong Yun. / Microfluidics-generated pancreatic islet microfibers for enhanced immunoprotection. In: Biomaterials. 2013 ; Vol. 34, No. 33. pp. 8122-8130.
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