Microglial activation and tyrosine hydroxylase immunoreactivity in the substantia nigral region following transient focal ischemia in rats

Youngbuhm Huh, Ji Wook Jung, Chan Park, Jae Ryun Ryu, Chan Young Shin, Won Ki Kim, Jong Hoon Ryu

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The temporal profiles of the changes of dopaminergic cells and microglial activation induced by transient cerebral ischemia were investigated in the substantia nigra pars compacta (SNc) located outside ischemic areas of rat brain. Transient cerebral ischemia was induced by intraluminal occlusion of the right middle cerebral artery for 2 h and reperfusion was continued for 1, 2, 3, 4, 7, 10, 14, 28, 60, and 120 days. Dopaminergic cells immunostained with tyrosine hydroxylase (TH)-antibody in the ipsilateral SNc were significantly decreased at 7 days post-ischemia compared with those in the contralateral side (P<0.05). However, at 60 and 120 days, there were no significant differences between ipsilateral and contralateral side of the SNc. Unlike the TH immunoreactivity, activated microglial cells immunostained with OX-42 antibody were significantly increased at 2 and 3 days and then decreased gradually until 10 days post-ischemia. Activated microglial cells were increased at 2 weeks post-ischemia, and this pattern remained until 60 days. These results suggest that the transient changes of TH-immunoreactive cells in the SNc caused by transient focal ischemia are correlated with a biphasic microglial cell activation response.

Original languageEnglish
Pages (from-to)63-67
Number of pages5
JournalNeuroscience Letters
Volume349
Issue number1
DOIs
Publication statusPublished - 2003 Sep 25
Externally publishedYes

Keywords

  • Ischemia
  • Microglia
  • Remote area
  • Substantia nigra
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Neuroscience(all)

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