Microinjection free delivery of miRNA inhibitor into zygotes

Jin Young Joo, Jonghwan Lee, Hae Young Ko, Yong Seung Lee, Do Hwan Lim, Eun Young Kim, Sujeong Cho, Ki Sung Hong, Jung Jae Ko, Suman Lee, Youngsik Lee, Youngsok Choi, Kyung Ah Lee, Soonhag Kim

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11 Citations (Scopus)

Abstract

The development of gene delivery systems into embryos is challenging due to technical difficulties, delivery efficiency and toxicity. Here, we developed an organic compound (VisuFect)-mediated gene delivery system for zygotes. The VisuFect, which is hydrophilic and Cy5.5-labeled, was conjugated with poly(A) oligo (VFA). The VFA into CHO cells showed clathrin-mediated internalization and no toxicity. The VFA successfully penetrated through the zona pellucida of fertilized eggs of various species including pigs, zebrafish, drosophilas and mice. The experiment with VisuFect-mediated delivery of the miR34c inhibitor showed similar results with direct microinjection of the miR34c inhibitor by suppressing the development of zygotes up to the blastocyst stage. Noticeable features of the VisuFect will provide great benefits for further studies on gene function in sperms and embryos.

Original languageEnglish
Article number5417
JournalScientific Reports
Volume4
DOIs
Publication statusPublished - 2014 Jun 24

ASJC Scopus subject areas

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    Joo, J. Y., Lee, J., Ko, H. Y., Lee, Y. S., Lim, D. H., Kim, E. Y., Cho, S., Hong, K. S., Ko, J. J., Lee, S., Lee, Y., Choi, Y., Lee, K. A., & Kim, S. (2014). Microinjection free delivery of miRNA inhibitor into zygotes. Scientific Reports, 4, [5417]. https://doi.org/10.1038/srep05417