Abstract
Resonant microcantilevers have recently enabled the label-free detection of biomolecules. Here, we observed the kinetics of biomolecular interactions such as antigen-antibody interactions and/or DNA hybridization based on a resonant frequency shift, obeying Langmuir kinetics, measured in a buffer solution. It is shown that the kinetics of DNA adsorptions on the surface is governed by intermolecular interactions between adsorbed DNA molecules. It is also shown that the kinetics of DNA hybridization is determined by the intermolecular interaction. It is implied that resonant microcantilever in buffer solution may allow for gaining insights into the kinetics of various molecular interactions.
| Original language | English |
|---|---|
| Article number | 173901 |
| Journal | Applied Physics Letters |
| Volume | 93 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - 2008 Nov 6 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Physics and Astronomy (miscellaneous)
Cite this
Micromechanical observation of the kinetics of biomolecular interactions. / Kwon, Taeyun; Eom, Kilho; Park, Jinsung; Yoon, Dae Sung; Lee, Hong Lim; Kim, Tae Song.
In: Applied Physics Letters, Vol. 93, No. 17, 173901, 06.11.2008.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Micromechanical observation of the kinetics of biomolecular interactions
AU - Kwon, Taeyun
AU - Eom, Kilho
AU - Park, Jinsung
AU - Yoon, Dae Sung
AU - Lee, Hong Lim
AU - Kim, Tae Song
PY - 2008/11/6
Y1 - 2008/11/6
N2 - Resonant microcantilevers have recently enabled the label-free detection of biomolecules. Here, we observed the kinetics of biomolecular interactions such as antigen-antibody interactions and/or DNA hybridization based on a resonant frequency shift, obeying Langmuir kinetics, measured in a buffer solution. It is shown that the kinetics of DNA adsorptions on the surface is governed by intermolecular interactions between adsorbed DNA molecules. It is also shown that the kinetics of DNA hybridization is determined by the intermolecular interaction. It is implied that resonant microcantilever in buffer solution may allow for gaining insights into the kinetics of various molecular interactions.
AB - Resonant microcantilevers have recently enabled the label-free detection of biomolecules. Here, we observed the kinetics of biomolecular interactions such as antigen-antibody interactions and/or DNA hybridization based on a resonant frequency shift, obeying Langmuir kinetics, measured in a buffer solution. It is shown that the kinetics of DNA adsorptions on the surface is governed by intermolecular interactions between adsorbed DNA molecules. It is also shown that the kinetics of DNA hybridization is determined by the intermolecular interaction. It is implied that resonant microcantilever in buffer solution may allow for gaining insights into the kinetics of various molecular interactions.
UR - http://www.scopus.com/inward/record.url?scp=55149102227&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=55149102227&partnerID=8YFLogxK
U2 - 10.1063/1.3006329
DO - 10.1063/1.3006329
M3 - Article
AN - SCOPUS:55149102227
VL - 93
JO - Applied Physics Letters
JF - Applied Physics Letters
SN - 0003-6951
IS - 17
M1 - 173901
ER -