Micromechanical observation of the kinetics of biomolecular interactions

Taeyun Kwon, Kilho Eom, Jinsung Park, Dae Sung Yoon, Hong Lim Lee, Tae Song Kim

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Resonant microcantilevers have recently enabled the label-free detection of biomolecules. Here, we observed the kinetics of biomolecular interactions such as antigen-antibody interactions and/or DNA hybridization based on a resonant frequency shift, obeying Langmuir kinetics, measured in a buffer solution. It is shown that the kinetics of DNA adsorptions on the surface is governed by intermolecular interactions between adsorbed DNA molecules. It is also shown that the kinetics of DNA hybridization is determined by the intermolecular interaction. It is implied that resonant microcantilever in buffer solution may allow for gaining insights into the kinetics of various molecular interactions.

Original languageEnglish
Article number173901
JournalApplied Physics Letters
Volume93
Issue number17
DOIs
Publication statusPublished - 2008

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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