Micropattened extracellular matrix protein chip for controlling the differentiation and migration of adult neural stem cells

S. Joo, J. Kim, E. Lee, N. Hong, Woong Sun, Y. Nam

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We applied soft-lithographic technique to design simple and reproducible laminin (LN)-polylysine cell culture substrates and investigated how adult neural stem cells (aNSCs) respond to the various spatial distribution of laminin, one of extracellular matrix (ECM) proteins enriched in the aNSC niche. We found that aNSC preferred to migrate and attach to LN stripes, and aNSC-derived neurons and astrocytes showed significant difference in motility towards LN stripes. By changing the spacing of LN stripes, we were able to control the alignment of neurons and astrocytes. Our findings would provide a deeper understanding in astrocyte-neuron interactions as well as ECM-stem cell interactions.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages665-667
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period15/10/2515/10/29

Keywords

  • Microcontact printing
  • Neural stem cell
  • Neuron

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Joo, S., Kim, J., Lee, E., Hong, N., Sun, W., & Nam, Y. (2015). Micropattened extracellular matrix protein chip for controlling the differentiation and migration of adult neural stem cells. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 665-667). Chemical and Biological Microsystems Society.