Micropattened extracellular matrix protein chip for controlling the differentiation and migration of adult neural stem cells

S. Joo; J. Kim; E. Lee; N. Hong; Woong Sun; Y. Nam

Micropattened extracellular matrix protein chip for controlling the differentiation and migration of adult neural stem cells

S. Joo, J. Kim, E. Lee, N. Hong, Woong Sun, Y. Nam

Department of Biomedical Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We applied soft-lithographic technique to design simple and reproducible laminin (LN)-polylysine cell culture substrates and investigated how adult neural stem cells (aNSCs) respond to the various spatial distribution of laminin, one of extracellular matrix (ECM) proteins enriched in the aNSC niche. We found that aNSC preferred to migrate and attach to LN stripes, and aNSC-derived neurons and astrocytes showed significant difference in motility towards LN stripes. By changing the spacing of LN stripes, we were able to control the alignment of neurons and astrocytes. Our findings would provide a deeper understanding in astrocyte-neuron interactions as well as ECM-stem cell interactions.

Original language	English
Title of host publication	MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	665-667
Number of pages	3
ISBN (Electronic)	9780979806483
Publication status	Published - 2015
Event	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 2015 Oct 25 → 2015 Oct 29

Other

Other	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Country	Korea, Republic of
City	Gyeongju
Period	15/10/25 → 15/10/29

Keywords

Microcontact printing
Neural stem cell
Neuron

ASJC Scopus subject areas

Control and Systems Engineering

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Micropattened extracellular matrix protein chip for controlling the differentiation and migration of adult neural stem cells. / Joo, S.; Kim, J.; Lee, E.; Hong, N.; Sun, Woong; Nam, Y.

MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. p. 665-667.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Joo, S, Kim, J, Lee, E, Hong, N, Sun, W & Nam, Y 2015, Micropattened extracellular matrix protein chip for controlling the differentiation and migration of adult neural stem cells. in MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 665-667, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, Gyeongju, Korea, Republic of, 15/10/25.

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abstract = "We applied soft-lithographic technique to design simple and reproducible laminin (LN)-polylysine cell culture substrates and investigated how adult neural stem cells (aNSCs) respond to the various spatial distribution of laminin, one of extracellular matrix (ECM) proteins enriched in the aNSC niche. We found that aNSC preferred to migrate and attach to LN stripes, and aNSC-derived neurons and astrocytes showed significant difference in motility towards LN stripes. By changing the spacing of LN stripes, we were able to control the alignment of neurons and astrocytes. Our findings would provide a deeper understanding in astrocyte-neuron interactions as well as ECM-stem cell interactions.",

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author = "S. Joo and J. Kim and E. Lee and N. Hong and Woong Sun and Y. Nam",

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booktitle = "MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

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AU - Joo, S.

AU - Kim, J.

AU - Lee, E.

AU - Hong, N.

AU - Sun, Woong

AU - Nam, Y.

PY - 2015

Y1 - 2015

N2 - We applied soft-lithographic technique to design simple and reproducible laminin (LN)-polylysine cell culture substrates and investigated how adult neural stem cells (aNSCs) respond to the various spatial distribution of laminin, one of extracellular matrix (ECM) proteins enriched in the aNSC niche. We found that aNSC preferred to migrate and attach to LN stripes, and aNSC-derived neurons and astrocytes showed significant difference in motility towards LN stripes. By changing the spacing of LN stripes, we were able to control the alignment of neurons and astrocytes. Our findings would provide a deeper understanding in astrocyte-neuron interactions as well as ECM-stem cell interactions.

AB - We applied soft-lithographic technique to design simple and reproducible laminin (LN)-polylysine cell culture substrates and investigated how adult neural stem cells (aNSCs) respond to the various spatial distribution of laminin, one of extracellular matrix (ECM) proteins enriched in the aNSC niche. We found that aNSC preferred to migrate and attach to LN stripes, and aNSC-derived neurons and astrocytes showed significant difference in motility towards LN stripes. By changing the spacing of LN stripes, we were able to control the alignment of neurons and astrocytes. Our findings would provide a deeper understanding in astrocyte-neuron interactions as well as ECM-stem cell interactions.

KW - Microcontact printing

KW - Neural stem cell

KW - Neuron

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Y2 - 25 October 2015 through 29 October 2015

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