Abstract
We applied soft-lithographic technique to design simple and reproducible laminin (LN)-polylysine cell culture substrates and investigated how adult neural stem cells (aNSCs) respond to the various spatial distribution of laminin, one of extracellular matrix (ECM) proteins enriched in the aNSC niche. We found that aNSC preferred to migrate and attach to LN stripes, and aNSC-derived neurons and astrocytes showed significant difference in motility towards LN stripes. By changing the spacing of LN stripes, we were able to control the alignment of neurons and astrocytes. Our findings would provide a deeper understanding in astrocyte-neuron interactions as well as ECM-stem cell interactions.
| Original language | English |
|---|---|
| Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 665-667 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806483 |
| Publication status | Published - 2015 |
| Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 2015 Oct 25 → 2015 Oct 29 |
Other
| Other | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
|---|---|
| Country | Korea, Republic of |
| City | Gyeongju |
| Period | 15/10/25 → 15/10/29 |
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Keywords
- Microcontact printing
- Neural stem cell
- Neuron
ASJC Scopus subject areas
- Control and Systems Engineering
Cite this
Micropattened extracellular matrix protein chip for controlling the differentiation and migration of adult neural stem cells. / Joo, S.; Kim, J.; Lee, E.; Hong, N.; Sun, Woong; Nam, Y.
MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. p. 665-667.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Micropattened extracellular matrix protein chip for controlling the differentiation and migration of adult neural stem cells
AU - Joo, S.
AU - Kim, J.
AU - Lee, E.
AU - Hong, N.
AU - Sun, Woong
AU - Nam, Y.
PY - 2015
Y1 - 2015
N2 - We applied soft-lithographic technique to design simple and reproducible laminin (LN)-polylysine cell culture substrates and investigated how adult neural stem cells (aNSCs) respond to the various spatial distribution of laminin, one of extracellular matrix (ECM) proteins enriched in the aNSC niche. We found that aNSC preferred to migrate and attach to LN stripes, and aNSC-derived neurons and astrocytes showed significant difference in motility towards LN stripes. By changing the spacing of LN stripes, we were able to control the alignment of neurons and astrocytes. Our findings would provide a deeper understanding in astrocyte-neuron interactions as well as ECM-stem cell interactions.
AB - We applied soft-lithographic technique to design simple and reproducible laminin (LN)-polylysine cell culture substrates and investigated how adult neural stem cells (aNSCs) respond to the various spatial distribution of laminin, one of extracellular matrix (ECM) proteins enriched in the aNSC niche. We found that aNSC preferred to migrate and attach to LN stripes, and aNSC-derived neurons and astrocytes showed significant difference in motility towards LN stripes. By changing the spacing of LN stripes, we were able to control the alignment of neurons and astrocytes. Our findings would provide a deeper understanding in astrocyte-neuron interactions as well as ECM-stem cell interactions.
KW - Microcontact printing
KW - Neural stem cell
KW - Neuron
UR - http://www.scopus.com/inward/record.url?scp=84983335577&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84983335577&partnerID=8YFLogxK
M3 - Conference contribution
SP - 665
EP - 667
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
ER -