Micropillar-based microfluidic device to regulate neurite networks of uniform-sized neurospheres

Da Eun Kim, Jong Min Lee, Christian D. Ahrberg, Mohammed R. Shaker, Ju Hyun Lee, Woong Sun, Bong Geun Chung

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The inability of neurons to undergo mitosis renders damage to the central or peripheral nervous system. Neural stem cell therapy could provide a path for treating the neurodegenerative diseases. However, reliable and simple tools for the developing and testing neural stem cell therapy are still required. Here, we show the development of a micropillar-based microfluidic device to trap the uniform-sized neurospheres. The neurospheres trapped within micropillar arrays were largely differentiated into neuronal cells, and their neurite networks were observed in the microfluidic device. Compared to conventional cultures on glass slides, the neurite networks generated with this method have a higher reproducibility. Furthermore, we demonstrated the effect of thapsigargin on the neurite networks in the microfluidic device, demonstrating that neural networks exposed to thapsigargin were largely diminished and disconnected from each other. Therefore, this micropillar-based microfluidic device could be a potential tool for screening of neurotoxins.

Original languageEnglish
JournalElectrophoresis
DOIs
Publication statusAccepted/In press - 2018 Jan 1

Keywords

  • Microfluidics
  • Micropillar array
  • Neurite network
  • Neurosphere
  • Thapsigargin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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  • Cite this

    Kim, D. E., Lee, J. M., Ahrberg, C. D., Shaker, M. R., Lee, J. H., Sun, W., & Chung, B. G. (Accepted/In press). Micropillar-based microfluidic device to regulate neurite networks of uniform-sized neurospheres. Electrophoresis. https://doi.org/10.1002/elps.201800119