Micropillar-based microfluidic device to regulate neurite networks of uniform-sized neurospheres

Da Eun Kim, Jong Min Lee, Christian D. Ahrberg, Mohammed R. Shaker, Ju Hyun Lee, Woong Sun, Bong Geun Chung

Research output: Contribution to journalArticle

Abstract

The inability of neurons to undergo mitosis renders damage to the central or peripheral nervous system. Neural stem cell therapy could provide a path for treating the neurodegenerative diseases. However, reliable and simple tools for the developing and testing neural stem cell therapy are still required. Here, we show the development of a micropillar-based microfluidic device to trap the uniform-sized neurospheres. The neurospheres trapped within micropillar arrays were largely differentiated into neuronal cells, and their neurite networks were observed in the microfluidic device. Compared to conventional cultures on glass slides, the neurite networks generated with this method have a higher reproducibility. Furthermore, we demonstrated the effect of thapsigargin on the neurite networks in the microfluidic device, demonstrating that neural networks exposed to thapsigargin were largely diminished and disconnected from each other. Therefore, this micropillar-based microfluidic device could be a potential tool for screening of neurotoxins.

Original languageEnglish
JournalElectrophoresis
DOIs
Publication statusAccepted/In press - 2018 Jan 1

Fingerprint

Lab-On-A-Chip Devices
Neurites
Microfluidics
Thapsigargin
Neural Stem Cells
Cell- and Tissue-Based Therapy
Stem cells
Neurodegenerative diseases
Peripheral Nervous System
Neurotoxins
Neurology
Mitosis
Neurodegenerative Diseases
Neurons
Glass
Screening
Central Nervous System
Neural networks
Testing

Keywords

  • Microfluidics
  • Micropillar array
  • Neurite network
  • Neurosphere
  • Thapsigargin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this

Kim, D. E., Lee, J. M., Ahrberg, C. D., Shaker, M. R., Lee, J. H., Sun, W., & Chung, B. G. (Accepted/In press). Micropillar-based microfluidic device to regulate neurite networks of uniform-sized neurospheres. Electrophoresis. https://doi.org/10.1002/elps.201800119

Micropillar-based microfluidic device to regulate neurite networks of uniform-sized neurospheres. / Kim, Da Eun; Lee, Jong Min; Ahrberg, Christian D.; Shaker, Mohammed R.; Lee, Ju Hyun; Sun, Woong; Chung, Bong Geun.

In: Electrophoresis, 01.01.2018.

Research output: Contribution to journalArticle

Kim, Da Eun ; Lee, Jong Min ; Ahrberg, Christian D. ; Shaker, Mohammed R. ; Lee, Ju Hyun ; Sun, Woong ; Chung, Bong Geun. / Micropillar-based microfluidic device to regulate neurite networks of uniform-sized neurospheres. In: Electrophoresis. 2018.
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