MicroRNA-199b-5p is involved in the Notch signaling pathway in osteosarcoma

Kyu Yeoun Won, Youn Wha Kim, Hyun Sook Kim, Seung Kwan Lee, Woon Won Jung, Yong Koo Park

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) play important roles in the development, differentiation, and function of different cell types and in the pathogenesis of various human diseases. miRNAs are differentially expressed in normal and cancer cells. The investigation of miRNA expression between healthy subjects and patients with osteosarcoma is crucial for future clinical trials. We performed miRNA microarray analysis on 8 formalin-fixed, paraffin-embedded osteosarcoma tissue samples. We confirmed the results of the microarray analysis using reverse transcription polymerase chain reaction. miRNA profiling of osteosarcoma tissue samples showed that expression of 10 miRNAs had increased 10-fold compared with normal controls. Among the 10 miRNAs, 3 miRNAs (miR-199b-5p, miR-338-3p, and miR-891a) were confirmed to have been up-regulated by reverse transcription polymerase chain reaction. After transfection of 4 osteosarcoma cell lines with miR-199b-5p inhibitor, the expression of Notch pathway components in the transfected cell lines was changed. These results revealed that miR-199b-5p plays a role in Notch signaling in osteosarcoma. Recently, the inhibition of Notch and HES1 signaling has been suggested as a potential therapeutic strategy to prevent metastasis in human osteosarcoma. Taken together with our results, we suggest that miR-199b-5p inhibitor may also be a therapeutic option for osteosarcoma.

Original languageEnglish
Pages (from-to)1648-1655
Number of pages8
JournalHuman Pathology
Volume44
Issue number8
DOIs
Publication statusPublished - 2013 Aug 1

Fingerprint

Osteosarcoma
MicroRNAs
Microarray Analysis
Reverse Transcription
Cell Line
Polymerase Chain Reaction
Paraffin
Formaldehyde
Transfection
Healthy Volunteers
Clinical Trials
Neoplasm Metastasis
Therapeutics

Keywords

  • MicroRNA
  • miR-199b-5p
  • Notch pathway
  • Osteosarcoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

MicroRNA-199b-5p is involved in the Notch signaling pathway in osteosarcoma. / Won, Kyu Yeoun; Kim, Youn Wha; Kim, Hyun Sook; Lee, Seung Kwan; Jung, Woon Won; Park, Yong Koo.

In: Human Pathology, Vol. 44, No. 8, 01.08.2013, p. 1648-1655.

Research output: Contribution to journalArticle

Won, Kyu Yeoun ; Kim, Youn Wha ; Kim, Hyun Sook ; Lee, Seung Kwan ; Jung, Woon Won ; Park, Yong Koo. / MicroRNA-199b-5p is involved in the Notch signaling pathway in osteosarcoma. In: Human Pathology. 2013 ; Vol. 44, No. 8. pp. 1648-1655.
@article{fd4d21b3b3b14d9fac36c66248548fbe,
title = "MicroRNA-199b-5p is involved in the Notch signaling pathway in osteosarcoma",
abstract = "MicroRNAs (miRNAs) play important roles in the development, differentiation, and function of different cell types and in the pathogenesis of various human diseases. miRNAs are differentially expressed in normal and cancer cells. The investigation of miRNA expression between healthy subjects and patients with osteosarcoma is crucial for future clinical trials. We performed miRNA microarray analysis on 8 formalin-fixed, paraffin-embedded osteosarcoma tissue samples. We confirmed the results of the microarray analysis using reverse transcription polymerase chain reaction. miRNA profiling of osteosarcoma tissue samples showed that expression of 10 miRNAs had increased 10-fold compared with normal controls. Among the 10 miRNAs, 3 miRNAs (miR-199b-5p, miR-338-3p, and miR-891a) were confirmed to have been up-regulated by reverse transcription polymerase chain reaction. After transfection of 4 osteosarcoma cell lines with miR-199b-5p inhibitor, the expression of Notch pathway components in the transfected cell lines was changed. These results revealed that miR-199b-5p plays a role in Notch signaling in osteosarcoma. Recently, the inhibition of Notch and HES1 signaling has been suggested as a potential therapeutic strategy to prevent metastasis in human osteosarcoma. Taken together with our results, we suggest that miR-199b-5p inhibitor may also be a therapeutic option for osteosarcoma.",
keywords = "MicroRNA, miR-199b-5p, Notch pathway, Osteosarcoma",
author = "Won, {Kyu Yeoun} and Kim, {Youn Wha} and Kim, {Hyun Sook} and Lee, {Seung Kwan} and Jung, {Woon Won} and Park, {Yong Koo}",
year = "2013",
month = "8",
day = "1",
doi = "10.1016/j.humpath.2013.01.016",
language = "English",
volume = "44",
pages = "1648--1655",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",
number = "8",

}

TY - JOUR

T1 - MicroRNA-199b-5p is involved in the Notch signaling pathway in osteosarcoma

AU - Won, Kyu Yeoun

AU - Kim, Youn Wha

AU - Kim, Hyun Sook

AU - Lee, Seung Kwan

AU - Jung, Woon Won

AU - Park, Yong Koo

PY - 2013/8/1

Y1 - 2013/8/1

N2 - MicroRNAs (miRNAs) play important roles in the development, differentiation, and function of different cell types and in the pathogenesis of various human diseases. miRNAs are differentially expressed in normal and cancer cells. The investigation of miRNA expression between healthy subjects and patients with osteosarcoma is crucial for future clinical trials. We performed miRNA microarray analysis on 8 formalin-fixed, paraffin-embedded osteosarcoma tissue samples. We confirmed the results of the microarray analysis using reverse transcription polymerase chain reaction. miRNA profiling of osteosarcoma tissue samples showed that expression of 10 miRNAs had increased 10-fold compared with normal controls. Among the 10 miRNAs, 3 miRNAs (miR-199b-5p, miR-338-3p, and miR-891a) were confirmed to have been up-regulated by reverse transcription polymerase chain reaction. After transfection of 4 osteosarcoma cell lines with miR-199b-5p inhibitor, the expression of Notch pathway components in the transfected cell lines was changed. These results revealed that miR-199b-5p plays a role in Notch signaling in osteosarcoma. Recently, the inhibition of Notch and HES1 signaling has been suggested as a potential therapeutic strategy to prevent metastasis in human osteosarcoma. Taken together with our results, we suggest that miR-199b-5p inhibitor may also be a therapeutic option for osteosarcoma.

AB - MicroRNAs (miRNAs) play important roles in the development, differentiation, and function of different cell types and in the pathogenesis of various human diseases. miRNAs are differentially expressed in normal and cancer cells. The investigation of miRNA expression between healthy subjects and patients with osteosarcoma is crucial for future clinical trials. We performed miRNA microarray analysis on 8 formalin-fixed, paraffin-embedded osteosarcoma tissue samples. We confirmed the results of the microarray analysis using reverse transcription polymerase chain reaction. miRNA profiling of osteosarcoma tissue samples showed that expression of 10 miRNAs had increased 10-fold compared with normal controls. Among the 10 miRNAs, 3 miRNAs (miR-199b-5p, miR-338-3p, and miR-891a) were confirmed to have been up-regulated by reverse transcription polymerase chain reaction. After transfection of 4 osteosarcoma cell lines with miR-199b-5p inhibitor, the expression of Notch pathway components in the transfected cell lines was changed. These results revealed that miR-199b-5p plays a role in Notch signaling in osteosarcoma. Recently, the inhibition of Notch and HES1 signaling has been suggested as a potential therapeutic strategy to prevent metastasis in human osteosarcoma. Taken together with our results, we suggest that miR-199b-5p inhibitor may also be a therapeutic option for osteosarcoma.

KW - MicroRNA

KW - miR-199b-5p

KW - Notch pathway

KW - Osteosarcoma

UR - http://www.scopus.com/inward/record.url?scp=84880326050&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84880326050&partnerID=8YFLogxK

U2 - 10.1016/j.humpath.2013.01.016

DO - 10.1016/j.humpath.2013.01.016

M3 - Article

C2 - 23574781

AN - SCOPUS:84880326050

VL - 44

SP - 1648

EP - 1655

JO - Human Pathology

JF - Human Pathology

SN - 0046-8177

IS - 8

ER -