MicroRNA-205-5p is upregulated in myelodysplastic syndromes and induces cell proliferation via PTEN suppression

Sook Jin Jang, In Sun Choi, Geon Park, Dae Soo Moon, Ji Seon Choi, Myung-Hyun Nam, Soo-Young Yoon, Cheol Hee Choi, Seong Ho Kang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Micro (mi)RNA dysregulation is implicated in the development of myelodysplastic syndrome (MDS). Chromosomal abnormalities on 1q are frequently detected in Korean patients with MDS; however, how these are related to disease development is unknown. The present study compared the expression profiles of miRNAs encoded by chromosome 1q between 65 MDS patients and 11 controls. We found that miR-205-5p levels were 12.5 fold higher in the former (P = 0.001). miR-205-5p level was increased in 44.7% of patients when an arbitrary 2−ΔCt cut-off value of 1.25 was used. miR-205-5p expression data were used to generate a receiver operating characteristic (ROC) curve for miR-205-5p, for which the area under the curve (AUC) was 0.825 (95% confidence interval: 0.710–0.941; P = 0.001). Moreover, transfection with a miR-205-5p mimic induced cell proliferation by inhibiting the expression of the tumor suppressor protein phosphatase and tensin homolog (PTEN). Our findings suggest that miR-205-5p upregulation contributes to MDS by suppressing PTEN and that miR-205-5p thus acts as an oncogene in hematopoietic cells.

Original languageEnglish
Pages (from-to)172-177
Number of pages6
JournalLeukemia Research
Volume47
DOIs
Publication statusPublished - 2016 Aug 1

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Keywords

  • Diagnosis
  • MDS
  • miR-205-5p
  • Oncogene
  • Upregulation

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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