MicroRNA miR-252 targets mbt to control the developmental growth of Drosophila

D. H. Lim, S. Lee, J. Y. Han, M. S. Choi, J. S. Hong, Youngsik Lee

Research output: Contribution to journalArticle

Abstract

Developmental growth is an intricate process involving the coordinated regulation of the expression of various genes, and microRNAs (miRNAs) play crucial roles in diverse processes throughout animal development. The ecdysone-responsive miRNA, miR-252, is normally upregulated during the pupal and adult stages of Drosophila development. Here, we found that overexpression of miR-252 in the larval fat body decreased total tissue mass through a reduction in both cell size and cell number, causing a concomitant decrease in larval size. Furthermore, miR-252 overexpression led to a delayed larval-to-pupal transition with defective anterior spiracle eversion, as well as a decrease in adult size and mass. Conversely, adult flies lacking miR-252 showed an increase in mass compared with control flies. We found that miR-252 directly targeted mbt, encoding a p21-activated kinase, to repress its expression. Notably, co-overexpression of mbt rescued the developmental and growth defects associated with miR-252 overexpression, indicating that mbt is a biologically relevant target of miR-252. Overall, our data support a role for the ecdysone/miR-252/mbt regulatory axis in growth control during Drosophila development.

Original languageEnglish
Pages (from-to)444-454
Number of pages11
JournalInsect Molecular Biology
Volume28
Issue number3
DOIs
Publication statusPublished - 2019 Jun 1

Fingerprint

MicroRNAs
microRNA
Drosophila
Ecdysone
ecdysone
Diptera
Growth
p21-Activated Kinases
spiracles
animal development
Fat Body
gene expression regulation
Gene Expression Regulation
fat body
Cell Size
phosphotransferases (kinases)
Cell Count
cells

Keywords

  • Drosophila
  • growth
  • mbt
  • miR-252

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

Cite this

MicroRNA miR-252 targets mbt to control the developmental growth of Drosophila. / Lim, D. H.; Lee, S.; Han, J. Y.; Choi, M. S.; Hong, J. S.; Lee, Youngsik.

In: Insect Molecular Biology, Vol. 28, No. 3, 01.06.2019, p. 444-454.

Research output: Contribution to journalArticle

Lim, D. H. ; Lee, S. ; Han, J. Y. ; Choi, M. S. ; Hong, J. S. ; Lee, Youngsik. / MicroRNA miR-252 targets mbt to control the developmental growth of Drosophila. In: Insect Molecular Biology. 2019 ; Vol. 28, No. 3. pp. 444-454.
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