MicroRNA response of inhalation exposure to hexanal in lung tissues from Fischer 344 rats

Yoon Cho, Mi Kyung Song, Seung Chan Jeong, Kyuhong Lee, Yongju Heo, Tae Sung Kim, Jae Chun Ryu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In previous studies, we have investigated the relationships between environmental chemicals and health risk based on omics analysis and identified significant biomarkers. Our current findings indicate that hexanal may be an important toxicant of the pulmonary system in epigenetic insights. MicroRNA (miRNA) is an important indicator of biomedical risk assessment and target identification. Hexanal is highly detectable in the exhaled breath of patients with chronic obstructive pulmonary disease (COPD) and chronic inflammatory lung disease. In this study, we aimed to identify hexanal-characterized miRNA-mRNA correlations involved in lung toxicity. Microarray analysis identified 56 miRNAs that commonly changed their expression more than 1.3-fold in three doses (600, 1000, and 1500 ppm) within hexanal-exposed Fischer 344 rats by inhalation, and 226 genes were predicted to be target genes of miRNAs through TargetScan analysis. By integrating analyses of miRNA and mRNA expression profiles, we identified one anti-correlated target gene (Chga; chromogranin A; parathyroid secretory protein 1). Comparative toxicogenomics database (CTD) analysis of this gene showed that Chga is involved with several disease categories such as cancer, respiratory tract disease, nervous system disease, and cardiovascular disease. Further research is necessary to elucidate the mechanisms of hexanal-responsive toxicologic pathways at the molecular level. This study concludes that our integrated approach to miRNA and mRNA enables us to identify molecular events in disease development induced by hexanal in an in vivo rat model.

Original languageEnglish
JournalEnvironmental Toxicology
DOIs
Publication statusAccepted/In press - 2015

Fingerprint

Inhalation Exposure
Inbred F344 Rats
MicroRNAs
Rats
Tissue
Lung
Pulmonary diseases
Genes
gene
Chromogranin A
Messenger RNA
Toxicogenetics
cardiovascular disease
Respiratory Tract Diseases
nervous system
integrated approach
Environmental Health
Health risks
health risk
Neurology

Keywords

  • Chga
  • Fischer 344 rat
  • Hexanal
  • Lung
  • MicroRNA

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Management, Monitoring, Policy and Law

Cite this

MicroRNA response of inhalation exposure to hexanal in lung tissues from Fischer 344 rats. / Cho, Yoon; Song, Mi Kyung; Jeong, Seung Chan; Lee, Kyuhong; Heo, Yongju; Kim, Tae Sung; Ryu, Jae Chun.

In: Environmental Toxicology, 2015.

Research output: Contribution to journalArticle

Cho, Yoon ; Song, Mi Kyung ; Jeong, Seung Chan ; Lee, Kyuhong ; Heo, Yongju ; Kim, Tae Sung ; Ryu, Jae Chun. / MicroRNA response of inhalation exposure to hexanal in lung tissues from Fischer 344 rats. In: Environmental Toxicology. 2015.
@article{8d7598d01d8949fcaeef64009438eca9,
title = "MicroRNA response of inhalation exposure to hexanal in lung tissues from Fischer 344 rats",
abstract = "In previous studies, we have investigated the relationships between environmental chemicals and health risk based on omics analysis and identified significant biomarkers. Our current findings indicate that hexanal may be an important toxicant of the pulmonary system in epigenetic insights. MicroRNA (miRNA) is an important indicator of biomedical risk assessment and target identification. Hexanal is highly detectable in the exhaled breath of patients with chronic obstructive pulmonary disease (COPD) and chronic inflammatory lung disease. In this study, we aimed to identify hexanal-characterized miRNA-mRNA correlations involved in lung toxicity. Microarray analysis identified 56 miRNAs that commonly changed their expression more than 1.3-fold in three doses (600, 1000, and 1500 ppm) within hexanal-exposed Fischer 344 rats by inhalation, and 226 genes were predicted to be target genes of miRNAs through TargetScan analysis. By integrating analyses of miRNA and mRNA expression profiles, we identified one anti-correlated target gene (Chga; chromogranin A; parathyroid secretory protein 1). Comparative toxicogenomics database (CTD) analysis of this gene showed that Chga is involved with several disease categories such as cancer, respiratory tract disease, nervous system disease, and cardiovascular disease. Further research is necessary to elucidate the mechanisms of hexanal-responsive toxicologic pathways at the molecular level. This study concludes that our integrated approach to miRNA and mRNA enables us to identify molecular events in disease development induced by hexanal in an in vivo rat model.",
keywords = "Chga, Fischer 344 rat, Hexanal, Lung, MicroRNA",
author = "Yoon Cho and Song, {Mi Kyung} and Jeong, {Seung Chan} and Kyuhong Lee and Yongju Heo and Kim, {Tae Sung} and Ryu, {Jae Chun}",
year = "2015",
doi = "10.1002/tox.22192",
language = "English",
journal = "Environmental Toxicology",
issn = "1520-4081",
publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - MicroRNA response of inhalation exposure to hexanal in lung tissues from Fischer 344 rats

AU - Cho, Yoon

AU - Song, Mi Kyung

AU - Jeong, Seung Chan

AU - Lee, Kyuhong

AU - Heo, Yongju

AU - Kim, Tae Sung

AU - Ryu, Jae Chun

PY - 2015

Y1 - 2015

N2 - In previous studies, we have investigated the relationships between environmental chemicals and health risk based on omics analysis and identified significant biomarkers. Our current findings indicate that hexanal may be an important toxicant of the pulmonary system in epigenetic insights. MicroRNA (miRNA) is an important indicator of biomedical risk assessment and target identification. Hexanal is highly detectable in the exhaled breath of patients with chronic obstructive pulmonary disease (COPD) and chronic inflammatory lung disease. In this study, we aimed to identify hexanal-characterized miRNA-mRNA correlations involved in lung toxicity. Microarray analysis identified 56 miRNAs that commonly changed their expression more than 1.3-fold in three doses (600, 1000, and 1500 ppm) within hexanal-exposed Fischer 344 rats by inhalation, and 226 genes were predicted to be target genes of miRNAs through TargetScan analysis. By integrating analyses of miRNA and mRNA expression profiles, we identified one anti-correlated target gene (Chga; chromogranin A; parathyroid secretory protein 1). Comparative toxicogenomics database (CTD) analysis of this gene showed that Chga is involved with several disease categories such as cancer, respiratory tract disease, nervous system disease, and cardiovascular disease. Further research is necessary to elucidate the mechanisms of hexanal-responsive toxicologic pathways at the molecular level. This study concludes that our integrated approach to miRNA and mRNA enables us to identify molecular events in disease development induced by hexanal in an in vivo rat model.

AB - In previous studies, we have investigated the relationships between environmental chemicals and health risk based on omics analysis and identified significant biomarkers. Our current findings indicate that hexanal may be an important toxicant of the pulmonary system in epigenetic insights. MicroRNA (miRNA) is an important indicator of biomedical risk assessment and target identification. Hexanal is highly detectable in the exhaled breath of patients with chronic obstructive pulmonary disease (COPD) and chronic inflammatory lung disease. In this study, we aimed to identify hexanal-characterized miRNA-mRNA correlations involved in lung toxicity. Microarray analysis identified 56 miRNAs that commonly changed their expression more than 1.3-fold in three doses (600, 1000, and 1500 ppm) within hexanal-exposed Fischer 344 rats by inhalation, and 226 genes were predicted to be target genes of miRNAs through TargetScan analysis. By integrating analyses of miRNA and mRNA expression profiles, we identified one anti-correlated target gene (Chga; chromogranin A; parathyroid secretory protein 1). Comparative toxicogenomics database (CTD) analysis of this gene showed that Chga is involved with several disease categories such as cancer, respiratory tract disease, nervous system disease, and cardiovascular disease. Further research is necessary to elucidate the mechanisms of hexanal-responsive toxicologic pathways at the molecular level. This study concludes that our integrated approach to miRNA and mRNA enables us to identify molecular events in disease development induced by hexanal in an in vivo rat model.

KW - Chga

KW - Fischer 344 rat

KW - Hexanal

KW - Lung

KW - MicroRNA

UR - http://www.scopus.com/inward/record.url?scp=84945371019&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84945371019&partnerID=8YFLogxK

U2 - 10.1002/tox.22192

DO - 10.1002/tox.22192

M3 - Article

C2 - 26403475

AN - SCOPUS:84945371019

JO - Environmental Toxicology

JF - Environmental Toxicology

SN - 1520-4081

ER -