MicroRNA/Argonaute 2 regulates nonsense-mediated messenger RNA decay

Junho Choe; Hana Cho; Hyung Chul Lee; Yoon Ki Kim

doi:10.1038/embor.2010.44

MicroRNA/Argonaute 2 regulates nonsense-mediated messenger RNA decay

Junho Choe, Hana Cho, Hyung Chul Lee, Yoon Ki Kim

Division of Life Sciences

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

Imperfect base-pairing between microRNA (miRNA) and the 3′-untranslated region of target messenger RNA (mRNA) triggers translational repression of the target mRNA. Here, we provide evidence that human Argonaute 2 targets cap-binding protein (CBP)80/20-bound mRNAs and exon junction complex-bound mRNAs and inhibits nonsense-mediated mRNA decay (NMD), which is restricted tightly to CBP80/20-bound mRNAs. Furthermore, microarray analyses reveal that a subset of cellular transcripts, which are expected to be targeted for NMD, is stabilized by miRNA-mediated gene silencing. The regulation of NMD by miRNAs will shed light on a new post-transcriptional regulation mechanism of gene expression in mammalian cells.

Original language	English
Pages (from-to)	380-386
Number of pages	7
Journal	EMBO Reports
Volume	11
Issue number	5
DOIs	https://doi.org/10.1038/embor.2010.44
Publication status	Published - 2010 May

Keywords

Ago2
CBP80/20
EIF4E
MicroRNA
NMD

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics

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