MicroRNA/Argonaute 2 regulates nonsense-mediated messenger RNA decay

Junho Choe, Hana Cho, Hyung Chul Lee, Yoon Ki Kim

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Imperfect base-pairing between microRNA (miRNA) and the 3′-untranslated region of target messenger RNA (mRNA) triggers translational repression of the target mRNA. Here, we provide evidence that human Argonaute 2 targets cap-binding protein (CBP)80/20-bound mRNAs and exon junction complex-bound mRNAs and inhibits nonsense-mediated mRNA decay (NMD), which is restricted tightly to CBP80/20-bound mRNAs. Furthermore, microarray analyses reveal that a subset of cellular transcripts, which are expected to be targeted for NMD, is stabilized by miRNA-mediated gene silencing. The regulation of NMD by miRNAs will shed light on a new post-transcriptional regulation mechanism of gene expression in mammalian cells.

Original languageEnglish
Pages (from-to)380-386
Number of pages7
JournalEMBO Reports
Volume11
Issue number5
DOIs
Publication statusPublished - 2010 May

Keywords

  • Ago2
  • CBP80/20
  • EIF4E
  • MicroRNA
  • NMD

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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