Abstract
Imperfect base-pairing between microRNA (miRNA) and the 3′-untranslated region of target messenger RNA (mRNA) triggers translational repression of the target mRNA. Here, we provide evidence that human Argonaute 2 targets cap-binding protein (CBP)80/20-bound mRNAs and exon junction complex-bound mRNAs and inhibits nonsense-mediated mRNA decay (NMD), which is restricted tightly to CBP80/20-bound mRNAs. Furthermore, microarray analyses reveal that a subset of cellular transcripts, which are expected to be targeted for NMD, is stabilized by miRNA-mediated gene silencing. The regulation of NMD by miRNAs will shed light on a new post-transcriptional regulation mechanism of gene expression in mammalian cells.
| Original language | English |
|---|---|
| Pages (from-to) | 380-386 |
| Number of pages | 7 |
| Journal | EMBO Reports |
| Volume | 11 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2010 May |
Keywords
- Ago2
- CBP80/20
- EIF4E
- MicroRNA
- NMD
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Genetics
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Choe, J., Cho, H., Lee, H. C., & Kim, Y. K. (2010). MicroRNA/Argonaute 2 regulates nonsense-mediated messenger RNA decay. EMBO Reports, 11(5), 380-386. https://doi.org/10.1038/embor.2010.44