Microwave-induced formation of oligomeric amyloid aggregates

Wonseok Lee, Yeseong Choi, Sang Won Lee, Insu Kim, Dongtak Lee, Yoochan Hong, Gyudo Lee, Dae Sung Yoon

Research output: Contribution to journalArticle

Abstract

Amyloid aggregates have emerged as a significant hallmark of neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. Although it has been recently reported that microwave heating induces amyloid aggregation compared with conventional heating methods, the mechanism of amyloid aggregate induction has remained unclear. In this study, we investigated the formation of oligomeric amyloid aggregates (OAAs) by microwave irradiation at microscale volumes of solution. Microwave irradiation of protein monomer solution triggered rapid formation of OAAs within 7 min. We characterized the formation of OAAs using atomic force microscopy, thioflavin T fluorescent assay and circular dichroism. In the microwave system, we also investigated the inhibitory effect on the formation of amyloid aggregates by L-ascorbic acid as well as enhanced amyloid aggregation by silver nanomaterials such as nanoparticles and nanowires. We believe that microwave technology has the potential to facilitate the study of amyloid aggregation in the presence of chemical agents or nanomaterials.

Original languageEnglish
Article number345604
JournalNanotechnology
Volume29
Issue number34
DOIs
Publication statusPublished - 2018 Jun 18

Fingerprint

Microwaves
Amyloid
Microwave irradiation
Agglomeration
Nanostructures
Nanostructured materials
Heating
Nanowires
Neurodegenerative diseases
Microwave heating
Atomic Force Microscopy
Ascorbic acid
Dichroism
Circular Dichroism
Silver
Neurodegenerative Diseases
Nanoparticles
Ascorbic Acid
Parkinson Disease
Assays

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Lee, W., Choi, Y., Lee, S. W., Kim, I., Lee, D., Hong, Y., ... Yoon, D. S. (2018). Microwave-induced formation of oligomeric amyloid aggregates. Nanotechnology, 29(34), [345604]. https://doi.org/10.1088/1361-6528/aac8f4

Microwave-induced formation of oligomeric amyloid aggregates. / Lee, Wonseok; Choi, Yeseong; Lee, Sang Won; Kim, Insu; Lee, Dongtak; Hong, Yoochan; Lee, Gyudo; Yoon, Dae Sung.

In: Nanotechnology, Vol. 29, No. 34, 345604, 18.06.2018.

Research output: Contribution to journalArticle

Lee, W, Choi, Y, Lee, SW, Kim, I, Lee, D, Hong, Y, Lee, G & Yoon, DS 2018, 'Microwave-induced formation of oligomeric amyloid aggregates', Nanotechnology, vol. 29, no. 34, 345604. https://doi.org/10.1088/1361-6528/aac8f4
Lee W, Choi Y, Lee SW, Kim I, Lee D, Hong Y et al. Microwave-induced formation of oligomeric amyloid aggregates. Nanotechnology. 2018 Jun 18;29(34). 345604. https://doi.org/10.1088/1361-6528/aac8f4
Lee, Wonseok ; Choi, Yeseong ; Lee, Sang Won ; Kim, Insu ; Lee, Dongtak ; Hong, Yoochan ; Lee, Gyudo ; Yoon, Dae Sung. / Microwave-induced formation of oligomeric amyloid aggregates. In: Nanotechnology. 2018 ; Vol. 29, No. 34.
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