Mild hyperthermia-induced apoptosis is dependent on p53 in human lymphoid cells

Young R. Seo, Martin L. Smith, Sung Sik Han, Daryl W. Fairbairn, Kim L. O'Neill, Jae Chun Ryu

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Abstract

Mild hyperthermia is known to enhance apoptosis. The p53 tumor- suppressor gene product has been shown to function in apoptosis in response to genotoxic stress. However, there is little information regarding the mechanism of p53-dependent apoptosis induced by heat stress. In present study, a p53 contribution in mild hyperthermia-induced apoptosis was investigated in human lymphoid system. After 30-minute exposure at 44°C, the accumulation of p53 protein was clearly observed in TK6 and ML-1 cells. Using comet assay, the more significant sensitivity to hyperthermic apoptosis was found in TK6 (wild-type p53) than in WI-L2-NS (mutated in p53). Furthermore, the significantly rapid shifting from early apoptotic phase to late apoptotic was observed in heat-induced p53 TK6 cells. These findings suggest that p53- dependent apoptosis is efficaciously induced by mild hyperthermia as non- genotoxic stress in human lymphoid system.

Original languageEnglish
Pages (from-to)285-292
Number of pages8
JournalResearch Communications in Molecular Pathology and Pharmacology
Volume104
Issue number3
Publication statusPublished - 1999 Dec 1

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

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