Miniaturized exosome isolation system using cationic polymer and syringe filter

Chanhee Park, Jinhyun Kim, Hoyoon Lee, Jina Choi, Hyunsung Kim, Sehyun Shin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Nano-sized exosome (30-150 nm) is emerging biomarker for liquid biopsy of cancer. However, the conventional method to isolate exosome: ultracentrifugation has various disadvantages such as time consuming (> 6 hr), high cost, etc. Here, we present a novel exosome isolation method using cationic polymer in a miniaturized system including a syringe filter. The present system can isolate exosome in 20 min with 20% higher vesicle yield and comparable exosomal miRNA levels compared to ultracentrifugation.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages1600-1601
Number of pages2
ISBN (Electronic)9781733419000
Publication statusPublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: 2019 Oct 272019 Oct 31

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
CountrySwitzerland
CityBasel
Period19/10/2719/10/31

Keywords

  • Exosome
  • Extracellular vesicle
  • Isolation
  • Polymer

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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