Miniaturized thermocycler based on thermoelectric heating for diagnosis of sexually transmitted disease by DNA amplification

Hyunjung Lim, Ga Eun Jo, Kyong Soo Kim, Seung Min Back, Hyuk Choi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Sexually transmitted disease (STD) is among the most common infectious diseases; therefore, it is necessary to develop sensitive early diagnostic techniques. As the gold standard, polymerase chain reaction (PCR) has been most widely employed for STD diagnosis; however, PCR requires large and expensive instruments. In this study, miniaturized thermal cycler using Peltier modules was developed for the PCR analysis. In comparison with the conventional PCR instrument, the Peltier-based micro-PCR (P-mPCR) device developed in this study enables one to amplify and successfully distinguish between DNA of different sizes. Furthermore, by using the clinical vaginal sample collected with the vaginal swab and tampon, different kinds of STD bacteria could be detected with high accuracy (∼94.19%) and high sensitivity (∼95.6%). Therefore, the P-mPCR device will be applicable in STD diagnosis as well as the detection of other bacteria/viruses using DNA amplification in regions including those with limited resources.

Original languageEnglish
Number of pages1
JournalThe Review of scientific instruments
Volume88
Issue number5
DOIs
Publication statusPublished - 2017 May 1

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polymerase chain reaction
Polymerase chain reaction
Amplification
DNA
deoxyribonucleic acid
Heating
heating
bacteria
Bacteria
viruses
infectious diseases
Viruses
resources
modules
sensitivity

ASJC Scopus subject areas

  • Instrumentation

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Miniaturized thermocycler based on thermoelectric heating for diagnosis of sexually transmitted disease by DNA amplification. / Lim, Hyunjung; Jo, Ga Eun; Kim, Kyong Soo; Back, Seung Min; Choi, Hyuk.

In: The Review of scientific instruments, Vol. 88, No. 5, 01.05.2017.

Research output: Contribution to journalArticle

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