MIR-27a regulates the TGF-β signaling pathway by targeting SMAD2 and SMAD4 in lung cancer

Dong Kyu Chae, Eunmi Ban, Young Sook Yoo, Eunice Eunkyeong Kim, Ja-Hyun Baik, Eun Joo Song

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The transforming growth factor-β (TGF-β) signaling pathway is associated with carcinogenesis and various biological processes. SMAD2 and SMAD4, which are putative tumor suppressors, have an important role in TGF-β signaling. The aberrant expression of these genes is implicated in some cancers. However, the mechanisms of SMAD2 and SMAD4 dysregulation are poorly understood. In this study, we observed that miR-27a was upregulated in lung cancer cell lines and patients. In addition, SMAD2 and SMAD4 genes were identified as targets of miR-27a by several target prediction databases and experimental validation. Functional studies revealed that miR-27a overexpression decreased SMAD2 and SMAD4 mRNA and protein levels. Furthermore, miR-27a contributed to cell proliferation and invasion by inhibiting TGF-β-induced cell cycle arrest. These results suggest that miR-27a may function as an oncogene by regulating SMAD2 and SMAD4 in lung cancer. Thus, miR-27a may be a potential target for cancer therapy.

Original languageEnglish
JournalMolecular Carcinogenesis
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Transforming Growth Factors
Lung Neoplasms
Biological Phenomena
Neoplasms
Cell Cycle Checkpoints
Oncogenes
Carcinogenesis
Cell Proliferation
Databases
Gene Expression
Cell Line
Messenger RNA
Genes
Proteins
Therapeutics

Keywords

  • Lung cancer
  • MiR-27a
  • SMAD2
  • SMAD4
  • TGF-β

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

Cite this

MIR-27a regulates the TGF-β signaling pathway by targeting SMAD2 and SMAD4 in lung cancer. / Chae, Dong Kyu; Ban, Eunmi; Yoo, Young Sook; Kim, Eunice Eunkyeong; Baik, Ja-Hyun; Song, Eun Joo.

In: Molecular Carcinogenesis, 2017.

Research output: Contribution to journalArticle

Chae, Dong Kyu ; Ban, Eunmi ; Yoo, Young Sook ; Kim, Eunice Eunkyeong ; Baik, Ja-Hyun ; Song, Eun Joo. / MIR-27a regulates the TGF-β signaling pathway by targeting SMAD2 and SMAD4 in lung cancer. In: Molecular Carcinogenesis. 2017.
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