MiR-29b controls fetal mouse neurogenesis by regulating ICAT-mediated Wnt/β-catenin signaling

J. Shin, Y. Shin, S. M. Oh, H. Yang, W. J. Yu, J. P. Lee, S. O. Huh, S. H. Lee, Y. H. Suh, Seok Chung, H. S. Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

β-Catenin has been widely implicated in the regulation of mammalian development and cellular homeostasis. However, the mechanisms by which Wnt/β-catenin signaling components regulate physiological events during brain development remain undetermined. Inactivation of glycogen synthase kinase (GSK)-3β leads to β-catenin accumulation in the nucleus, where it couples with T-cell factor (TCF), an association that is disrupted by ICAT (inhibitor of β-catenin and T cell factor). In this study, we sought to determine whether regulation of ICAT by members of the microRNA-29 family plays a role during neurogenesis and whether deregulation of ICAT results in defective neurogenesis due to impaired β-catenin-mediated signaling. We found that miR-29b, but not miR-29a or 29c, is significantly upregulated in three-dimensionally cultured neural stem cells (NSCs), whereas ICAT is reduced as aged. Treatment with a miR-29b reduced the reporter activity of a luciferase-ICAT 3'-UTR construct whereas a control (scrambled) miRNA oligonucleotide did not, indicating that miR-29b directly targets the 3'-UTR of ICAT. We also found that treatment with miR-29b diminished NSC self-renewal and proliferation, and controlled their fate, directing their differentiation along certain cell lineages. Furthermore, our in vivo results showed that inhibition of miR-29b by in utero electroporation induced a profound defect in corticogenesis during mouse development. Taken together, our results demonstrate that miR-29b plays a pivotal role in fetal mouse neurogenesis by regulating ICAT-mediated Wnt/β-catenin signaling.

Original languageEnglish
Article numbere1473
JournalCell Death and Disease
Volume5
Issue number10
DOIs
Publication statusPublished - 2014 Jan 1

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Catenins
Neurogenesis
TCF Transcription Factors
Neural Stem Cells
3' Untranslated Regions
MicroRNAs
Glycogen Synthase Kinase 3
Electroporation
Cell Lineage
Luciferases
Oligonucleotides
Homeostasis
Brain

ASJC Scopus subject areas

  • Cell Biology
  • Immunology
  • Cancer Research
  • Cellular and Molecular Neuroscience

Cite this

Shin, J., Shin, Y., Oh, S. M., Yang, H., Yu, W. J., Lee, J. P., ... Kim, H. S. (2014). MiR-29b controls fetal mouse neurogenesis by regulating ICAT-mediated Wnt/β-catenin signaling. Cell Death and Disease, 5(10), [e1473]. https://doi.org/10.1038/cddis.2014.439

MiR-29b controls fetal mouse neurogenesis by regulating ICAT-mediated Wnt/β-catenin signaling. / Shin, J.; Shin, Y.; Oh, S. M.; Yang, H.; Yu, W. J.; Lee, J. P.; Huh, S. O.; Lee, S. H.; Suh, Y. H.; Chung, Seok; Kim, H. S.

In: Cell Death and Disease, Vol. 5, No. 10, e1473, 01.01.2014.

Research output: Contribution to journalArticle

Shin, J, Shin, Y, Oh, SM, Yang, H, Yu, WJ, Lee, JP, Huh, SO, Lee, SH, Suh, YH, Chung, S & Kim, HS 2014, 'MiR-29b controls fetal mouse neurogenesis by regulating ICAT-mediated Wnt/β-catenin signaling', Cell Death and Disease, vol. 5, no. 10, e1473. https://doi.org/10.1038/cddis.2014.439
Shin, J. ; Shin, Y. ; Oh, S. M. ; Yang, H. ; Yu, W. J. ; Lee, J. P. ; Huh, S. O. ; Lee, S. H. ; Suh, Y. H. ; Chung, Seok ; Kim, H. S. / MiR-29b controls fetal mouse neurogenesis by regulating ICAT-mediated Wnt/β-catenin signaling. In: Cell Death and Disease. 2014 ; Vol. 5, No. 10.
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