MiR-495 and miR-551a inhibit the migration and invasion of human gastric cancer cells by directly interacting with PRL-3

Zhengrong Li, Yi Cao, Zhigang Jie, Yi Liu, Yingliang Li, Junhe Li, Guoming Zhu, Zhengren Liu, Yi Tu, Gen Peng, Dong woo Lee, Sung soo Park

    Research output: Contribution to journalArticlepeer-review

    92 Citations (Scopus)

    Abstract

    The phosphatase of regenerating liver-3 (. PRL-. 3) gene is associated with metastasis in gastric cancer, and is believed to play a causative role by promoting tumor cell motility, invasion, and metastasis, but little is known of the mechanisms involved. We previously reported that . PRL-. 3 expression is significantly higher in the tissues of primary gastric carcinomas with peritoneal metastasis. In the present study, we found that two microRNAs (miRNAs), miR-495 and miR-551a, predicted to target . PRL-. 3, are downregulated in gastric carcinoma samples. The validation of this interaction between those two miRNAs and . PRL-. 3 was confirmed by western blotting and quantitative real-time PCR (qPCR) in GC cell lines transfected with miR-495 and miR-551a mimics. Furthermore, the migration and invasion of GC cells were significantly inhibited by transfection with miR-495 or -551a mimics, and the mRNA and protein levels of . PRL-. 3 were reduced in cells overexpressing miR-495 or -551a. Collectively, our findings suggest that miR-495 and miR-551a both act as tumor suppressors by targeting the . PRL-. 3 oncogene and inhibiting gastric cancer cell migration and invasion. The findings of this study contribute to current understanding of the functions of miRNA mimics in GC gene therapy.

    Original languageEnglish
    Pages (from-to)41-47
    Number of pages7
    JournalCancer letters
    Volume323
    Issue number1
    DOIs
    Publication statusPublished - 2012 Oct 1

    Keywords

    • Gastric carcinoma
    • MiR-495
    • MiR-551a
    • PRL-3

    ASJC Scopus subject areas

    • Oncology
    • Cancer Research

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