MiR-5003-3p promotes epithelial-mesenchymal transition in breast cancer cells through Snail stabilization and direct targeting of E-cadherin

Seo Young Kwak, Je Ok Yoo, Hyun Ju An, In Hwa Bae, Myung Jin Park, Joon Kim, Young Hoon Han

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

One of the initial steps in metastatic dissemination is the epithelial-mesenchymal transition (EMT). Along this line, microRNAs (miRNAs) have been shown to function as important regulators of tumor progression at various stages. Therefore, we performed a functional screening for EMT-regulating miRNAs and identified several candidate miRNAs. Among these, we demonstrated that miR-5003-3p induces cellular features characteristic of EMT. miR-5003-3p induced upregulation of Snail, a key EMT-promoting transcription factor and transcriptional repressor of E-cadherin, through protein stabilization. MDM2 was identified as a direct target of miR-5003-3p, the downregulation of which induced Snail stabilization. E-cadherin was also demonstrated to be a direct target of miR-5003-3p, reinforcing the EMT-promoting function of miR-5003-3p. In situ hybridization and immunohistochemical analyses using tissue microarrays revealed that miR-5003-3p expression was higher in paired metastatic breast carcinoma tissues than in primary ductal carcinoma tissues, and was inversely correlated with the expression of MDM2 and E-cadherin. Furthermore, miR-5003-3p enhanced the formation of metastatic nodules in the lungs of mice in a tail vein injection experiment. Collectively, our results suggest that miR-5003-3p functions as a metastasis activator by promoting EMT through dual regulation of Snail stability and E-cadherin, and may therefore be a potential therapeutic target in metastatic cancers.

Original languageEnglish
Pages (from-to)372-383
Number of pages12
JournalJournal of Molecular Cell Biology
Volume8
Issue number5
DOIs
Publication statusPublished - 2016 Oct 1

Fingerprint

Epithelial-Mesenchymal Transition
Snails
Cadherins
Breast Neoplasms
MicroRNAs
Tissue Array Analysis
Ductal Carcinoma
In Situ Hybridization
Tail
Veins
Neoplasms
Transcription Factors
Up-Regulation
Down-Regulation
Neoplasm Metastasis
Lung
Injections
Proteins

Keywords

  • E-cadherin
  • EMT
  • MDM2
  • metastasis
  • miR-5003-3p
  • Snail

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

MiR-5003-3p promotes epithelial-mesenchymal transition in breast cancer cells through Snail stabilization and direct targeting of E-cadherin. / Kwak, Seo Young; Yoo, Je Ok; An, Hyun Ju; Bae, In Hwa; Park, Myung Jin; Kim, Joon; Han, Young Hoon.

In: Journal of Molecular Cell Biology, Vol. 8, No. 5, 01.10.2016, p. 372-383.

Research output: Contribution to journalArticle

Kwak, Seo Young ; Yoo, Je Ok ; An, Hyun Ju ; Bae, In Hwa ; Park, Myung Jin ; Kim, Joon ; Han, Young Hoon. / MiR-5003-3p promotes epithelial-mesenchymal transition in breast cancer cells through Snail stabilization and direct targeting of E-cadherin. In: Journal of Molecular Cell Biology. 2016 ; Vol. 8, No. 5. pp. 372-383.
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