Misexpression screen delineates novel genes controlling Drosophila lifespan

Donggi Paik, Yeo Gil Jang, Young Eun Lee, Young Nam Lee, Rochelle Yamamoto, Heon Yung Gee, Seungmin Yoo, Eunkyung Bae, Kyung Jin Min, Marc Tatar, Joong Jean Park

    Research output: Contribution to journalArticlepeer-review

    36 Citations (Scopus)

    Abstract

    In an initial preliminary screen we identified factors associated with controlling . Drosophila aging by examining longevity in adults where EP elements induced over-expression or antisense-RNA at genes adjacent to each insertion. Here, we study 45 EP lines that initially showed at least 10% longer mean lifespan than controls. These 45 lines and a . daughterless (da)-Gal4 stock were isogenized into a . CS10 wild-type background. Sixteen EP lines corresponding to 15 genes significantly extended lifespan when their target genes were driven by . da-Gal4. In each case, the target genes were seen to be over-expressed. Independently derived UAS-gene transgenic stocks were available or made for two candidates: . ImpL2 which is ecdysone-inducible gene L2, and . CG33138, 1,4-alpha-glucan branching enzyme. With both, adult lifespan was increased upon over-expression via the GeneSwitch inducible Gal4 driver system. Several genes in this set of 15 correspond to previously discovered longevity assurance systems such as insulin/IGF-1 signaling, gene silencing, and autophagy; others suggest new potential mechanisms for the control of aging including mRNA synthesis and maturation, intracellular vesicle trafficking, and neuroendocrine regulation.

    Original languageEnglish
    Pages (from-to)234-245
    Number of pages12
    JournalMechanisms of Ageing and Development
    Volume133
    Issue number5
    DOIs
    Publication statusPublished - 2012 May

    Keywords

    • Aging
    • ImpL2
    • Longevity genes
    • Misexpression screen

    ASJC Scopus subject areas

    • Ageing
    • Developmental Biology

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