Abstract
In an initial preliminary screen we identified factors associated with controlling . Drosophila aging by examining longevity in adults where EP elements induced over-expression or antisense-RNA at genes adjacent to each insertion. Here, we study 45 EP lines that initially showed at least 10% longer mean lifespan than controls. These 45 lines and a . daughterless (da)-Gal4 stock were isogenized into a . CS10 wild-type background. Sixteen EP lines corresponding to 15 genes significantly extended lifespan when their target genes were driven by . da-Gal4. In each case, the target genes were seen to be over-expressed. Independently derived UAS-gene transgenic stocks were available or made for two candidates: . ImpL2 which is ecdysone-inducible gene L2, and . CG33138, 1,4-alpha-glucan branching enzyme. With both, adult lifespan was increased upon over-expression via the GeneSwitch inducible Gal4 driver system. Several genes in this set of 15 correspond to previously discovered longevity assurance systems such as insulin/IGF-1 signaling, gene silencing, and autophagy; others suggest new potential mechanisms for the control of aging including mRNA synthesis and maturation, intracellular vesicle trafficking, and neuroendocrine regulation.
| Original language | English |
|---|---|
| Pages (from-to) | 234-245 |
| Number of pages | 12 |
| Journal | Mechanisms of Ageing and Development |
| Volume | 133 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2012 May 1 |
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Keywords
- Aging
- ImpL2
- Longevity genes
- Misexpression screen
ASJC Scopus subject areas
- Ageing
- Developmental Biology
Cite this
Misexpression screen delineates novel genes controlling Drosophila lifespan. / Paik, Donggi; Jang, Yeo Gil; Lee, Young Eun; Lee, Young Nam; Yamamoto, Rochelle; Gee, Heon Yung; Yoo, Seungmin; Bae, Eunkyung; Min, Kyung Jin; Tatar, Marc; Park, Joong Jean.
In: Mechanisms of Ageing and Development, Vol. 133, No. 5, 01.05.2012, p. 234-245.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Misexpression screen delineates novel genes controlling Drosophila lifespan
AU - Paik, Donggi
AU - Jang, Yeo Gil
AU - Lee, Young Eun
AU - Lee, Young Nam
AU - Yamamoto, Rochelle
AU - Gee, Heon Yung
AU - Yoo, Seungmin
AU - Bae, Eunkyung
AU - Min, Kyung Jin
AU - Tatar, Marc
AU - Park, Joong Jean
PY - 2012/5/1
Y1 - 2012/5/1
N2 - In an initial preliminary screen we identified factors associated with controlling . Drosophila aging by examining longevity in adults where EP elements induced over-expression or antisense-RNA at genes adjacent to each insertion. Here, we study 45 EP lines that initially showed at least 10% longer mean lifespan than controls. These 45 lines and a . daughterless (da)-Gal4 stock were isogenized into a . CS10 wild-type background. Sixteen EP lines corresponding to 15 genes significantly extended lifespan when their target genes were driven by . da-Gal4. In each case, the target genes were seen to be over-expressed. Independently derived UAS-gene transgenic stocks were available or made for two candidates: . ImpL2 which is ecdysone-inducible gene L2, and . CG33138, 1,4-alpha-glucan branching enzyme. With both, adult lifespan was increased upon over-expression via the GeneSwitch inducible Gal4 driver system. Several genes in this set of 15 correspond to previously discovered longevity assurance systems such as insulin/IGF-1 signaling, gene silencing, and autophagy; others suggest new potential mechanisms for the control of aging including mRNA synthesis and maturation, intracellular vesicle trafficking, and neuroendocrine regulation.
AB - In an initial preliminary screen we identified factors associated with controlling . Drosophila aging by examining longevity in adults where EP elements induced over-expression or antisense-RNA at genes adjacent to each insertion. Here, we study 45 EP lines that initially showed at least 10% longer mean lifespan than controls. These 45 lines and a . daughterless (da)-Gal4 stock were isogenized into a . CS10 wild-type background. Sixteen EP lines corresponding to 15 genes significantly extended lifespan when their target genes were driven by . da-Gal4. In each case, the target genes were seen to be over-expressed. Independently derived UAS-gene transgenic stocks were available or made for two candidates: . ImpL2 which is ecdysone-inducible gene L2, and . CG33138, 1,4-alpha-glucan branching enzyme. With both, adult lifespan was increased upon over-expression via the GeneSwitch inducible Gal4 driver system. Several genes in this set of 15 correspond to previously discovered longevity assurance systems such as insulin/IGF-1 signaling, gene silencing, and autophagy; others suggest new potential mechanisms for the control of aging including mRNA synthesis and maturation, intracellular vesicle trafficking, and neuroendocrine regulation.
KW - Aging
KW - ImpL2
KW - Longevity genes
KW - Misexpression screen
UR - http://www.scopus.com/inward/record.url?scp=84862823352&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84862823352&partnerID=8YFLogxK
U2 - 10.1016/j.mad.2012.02.001
DO - 10.1016/j.mad.2012.02.001
M3 - Article
C2 - 22366109
AN - SCOPUS:84862823352
VL - 133
SP - 234
EP - 245
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
SN - 0047-6374
IS - 5
ER -