TY - JOUR
T1 - Misplacement of Purkinje cells during postnatal development in Bax Knock-Out mice
T2 - A novel role for programmed cell death in the nervous system?
AU - Jung, A. Rong
AU - Tae, Woo Kim
AU - Im, Joo Rhyu
AU - Kim, Hyun
AU - Young, Don Lee
AU - Vinsant, Sharon
AU - Oppenheim, Ronald W.
AU - Sun, Woong
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2008/3/12
Y1 - 2008/3/12
N2 - During early postnatal development, the orchestrated regulation of proliferation, migration and the survival versus elimination of neurons is essential for histogenesis of the cerebellum. For instance, Purkinje cells (PCs) promote the proliferation and migration of external granule cells (EGCs), whereas EGCs in turn play a role in the migration of PCs. Considering that a substantial number of neurons undergo programmed cell death (PCD) during cerebellar development, it seems likely that neuronal loss could have a significant role in the histogenesis of the cerebellum. To address this question, we examined postnatal development of the cerebellum in Bax-knock-out (KO) mice in which the PCD of PC has been reported to be selectively reduced or eliminated, whereas EGCs are unaffected. We confirmed the absence of PC PCD as well as the normal PCD of EGCs in Bax-KO mice. We also observed a subpopulation of PCs that were misplaced in the inner granule cell layer of Bax-KO mice on postnatal day 5 (P5) to P10 and that, by the end of the major period of cerebellar histogenesis (P14), lose expression of the PC marker calbindin. These results suggest that the removal of ectopically located neurons may be a previously unrecognized function of developmental PCD.
AB - During early postnatal development, the orchestrated regulation of proliferation, migration and the survival versus elimination of neurons is essential for histogenesis of the cerebellum. For instance, Purkinje cells (PCs) promote the proliferation and migration of external granule cells (EGCs), whereas EGCs in turn play a role in the migration of PCs. Considering that a substantial number of neurons undergo programmed cell death (PCD) during cerebellar development, it seems likely that neuronal loss could have a significant role in the histogenesis of the cerebellum. To address this question, we examined postnatal development of the cerebellum in Bax-knock-out (KO) mice in which the PCD of PC has been reported to be selectively reduced or eliminated, whereas EGCs are unaffected. We confirmed the absence of PC PCD as well as the normal PCD of EGCs in Bax-KO mice. We also observed a subpopulation of PCs that were misplaced in the inner granule cell layer of Bax-KO mice on postnatal day 5 (P5) to P10 and that, by the end of the major period of cerebellar histogenesis (P14), lose expression of the PC marker calbindin. These results suggest that the removal of ectopically located neurons may be a previously unrecognized function of developmental PCD.
KW - Bax
KW - Cerebellum
KW - Cerebral cortex
KW - Migration
KW - Programmed cell death
KW - Purkinje cell
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U2 - 10.1523/JNEUROSCI.3897-07.2008
DO - 10.1523/JNEUROSCI.3897-07.2008
M3 - Article
C2 - 18337425
AN - SCOPUS:40849120902
VL - 28
SP - 2941
EP - 2948
JO - Journal of Neuroscience
JF - Journal of Neuroscience
SN - 0270-6474
IS - 11
ER -