Mitochondria are impaired in the adipocytes of type 2 diabetic mice

H. J. Choo, Jae-Hong Kim, O. B. Kwon, C. S. Lee, J. Y. Mun, Sung Sik Han, Y. S. Yoon, G. Yoon, Kyung Mook Choi, Young-Gyu Ko

Research output: Contribution to journalArticle

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Abstract

Aims/hypothesis: The aim of this study was to confirm a link between mitochondrial dysfunction and type 2 diabetes. Materials and methods: Cellular levels of mitochondrial proteins, cellular mitochondrial DNA content, and mitochondrial function and morphology were assessed by MitoTracker staining and electron microscopy, in white adipose tissue of 12-week-old male wild-type, obese (ob/ob), and diabetic (db/db) mice. Results: Levels of mitochondrial proteins were found to be very similar in the livers and muscles of all the mice studied. However, levels were greatly decreased in the adipocytes of db/db mice, but not in those of the wild-type and ob/ob mice. Levels of mitochondrial DNA were also found to be considerably reduced in the adipocytes of db/db mice. MitoTracker staining and under electron microscopy revealed that the number of mitochondria was reduced in adipocytes of db/db mice. Respiration and fatty acid oxidation studies indicated mitochondrial dysfunction in adipocytes of db/db mice. Interestingly, there was an increase in mitochondria and mitochondrial protein production in adipocytes of db/db mice treated with rosiglitazone, an agent that enhances insulin sensitivity. Conclusions/interpretation: Taken together, these data indicate that mitochondrial loss in adipose tissue is correlated with the development of type 2 diabetes.

Original languageEnglish
Pages (from-to)784-791
Number of pages8
JournalDiabetologia
Volume49
Issue number4
DOIs
Publication statusPublished - 2006 Apr 1

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Adipocytes
Mitochondria
Mitochondrial Proteins
rosiglitazone
Mitochondrial DNA
Type 2 Diabetes Mellitus
Electron Microscopy
Staining and Labeling
White Adipose Tissue
Insulin Resistance
Adipose Tissue
Respiration
Fatty Acids
Muscles
Liver

Keywords

  • Adipocyte
  • Diabetes
  • Mitochondria
  • Rosiglitazone

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Mitochondria are impaired in the adipocytes of type 2 diabetic mice. / Choo, H. J.; Kim, Jae-Hong; Kwon, O. B.; Lee, C. S.; Mun, J. Y.; Han, Sung Sik; Yoon, Y. S.; Yoon, G.; Choi, Kyung Mook; Ko, Young-Gyu.

In: Diabetologia, Vol. 49, No. 4, 01.04.2006, p. 784-791.

Research output: Contribution to journalArticle

Choo, H. J. ; Kim, Jae-Hong ; Kwon, O. B. ; Lee, C. S. ; Mun, J. Y. ; Han, Sung Sik ; Yoon, Y. S. ; Yoon, G. ; Choi, Kyung Mook ; Ko, Young-Gyu. / Mitochondria are impaired in the adipocytes of type 2 diabetic mice. In: Diabetologia. 2006 ; Vol. 49, No. 4. pp. 784-791.
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