Mitochondria-immobilized ph-sensitive off-on fluorescent probe

Min Hee Lee, Nayoung Park, Chunsik Yi, Ji Hye Han, Ji Hye Hong, Kwang Pyo Kim, Dong Hoon Kang, Jonathan L. Sessler, Chulhun Kang, Jong Seung Kim

Research output: Contribution to journalArticle

244 Citations (Scopus)

Abstract

We report here a mitochondria-targetable pH-sensitive probe that allows for a quantitative measurement of mitochondrial pH changes, as well as the real-time monitoring of pH-related physiological effects in live cells. This system consists of a piperazine-linked naphthalimide as a fluorescence off-on signaling unit, a cationic triphenylphosphonium group for mitochondrial targeting, and a reactive benzyl chloride subunit for mitochondrial fixation. It operates well in a mitochondrial environment within whole cells and displays a desirable off-on fluorescence response to mitochondrial acidification. Moreover, this probe allows for the monitoring of impaired mitochondria undergoing mitophagic elimination as the result of nutrient starvation. It thus allows for the monitoring of the organelle-specific dynamics associated with the conversion between physiological and pathological states.

Original languageEnglish
Pages (from-to)14136-14142
Number of pages7
JournalJournal of the American Chemical Society
Volume136
Issue number40
DOIs
Publication statusPublished - 2014 Oct 8

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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    Lee, M. H., Park, N., Yi, C., Han, J. H., Hong, J. H., Kim, K. P., Kang, D. H., Sessler, J. L., Kang, C., & Kim, J. S. (2014). Mitochondria-immobilized ph-sensitive off-on fluorescent probe. Journal of the American Chemical Society, 136(40), 14136-14142. https://doi.org/10.1021/ja506301n