Mitochondrial H2Sn-Mediated Anti-Inflammatory Theranostics

Won Young Kim, Miae Won, Seyoung Koo, Xingcai Zhang, Jong Seung Kim

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The insistent demand for space-controllable delivery, which reduces the side effects of non-steroidal anti-inflammatory drugs (NSAIDs), has led to the development of a new theranostics-based approach for anti-inflammatory therapy. The current anti-inflammatory treatments can be improved by designing a drug delivery system responsive to the inflammatory site biomarker, hydrogen polysulfide (H2Sn). Here, we report a novel theranostic agent 1 (TA1), consisting of three parts: H2Sn-mediated triggering part, a two-photon fluorophore bearing mitochondria targeting unit (Rhodol-TPP), and anti-inflammatory COX inhibitor (indomethacin). In vitro experiments showed that TA1 selectively reacts with H2Sn to concomitantly release both Rhodol-TPP and indomethacin. Confocal-microscopy imaging of inflammation-induced live cells suggested that TA1 is localized in the mitochondria where the H2Sn is overexpressed. The TA1 reacted with H2Sn in the endogenous and exogenous H2Sn environments and in lipopolysaccharide treated inflammatory cells. Moreover, TA1 suppressed COX-2 level in the inflammatory-induced cells and prostaglandin E2 (PGE2) level in blood serum from inflammation-induced mouse models. In vivo experiments with inflammation-induced mouse models suggested that TA1 exhibits inflammation-site-elective drug release followed by significant therapeutic effects, showing its function as a theranostic agent, capable of both anti-inflammatory therapy and precise diagnosis. Theranostic behavior of TA1 is highly applicable in vivo model therapeutics for the inflammatory disease. [Figure not available: see fulltext.]

Original languageEnglish
Article number168
JournalNano-Micro Letters
Volume13
Issue number1
DOIs
Publication statusPublished - 2021 Dec

Keywords

  • Anti-inflammation
  • Hydrogen polysulfides
  • Mitochondria
  • Theranostics

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Electrical and Electronic Engineering

Fingerprint

Dive into the research topics of 'Mitochondrial H<sub>2</sub>S<sub>n</sub>-Mediated Anti-Inflammatory Theranostics'. Together they form a unique fingerprint.

Cite this