Mitochondrial oxidative phosphorylation complexes exist in the sarcolemma of skeletal muscle

Hyun Lee, Seung Hyeob Kim, Jae Seon Lee, Yun Hee Yang, Jwa Min Nam, Bong Woo Kim, Young-Gyu Ko

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Although proteomic analyses have revealed the presence of mitochondrial oxidative phosphorylation (OXPHOS) proteins in the plasma membrane, there have been no in-depth evaluations of the presence or function of OXPHOS I-V in the plasma membrane. Here, we demonstrate the in situ localization of OXPHOS I-V complexes to the sarcolemma of skeletal muscle by immunofluorescence and immunohistochemistry. A portion of the OXPHOS I-V complex proteins was not co-stained with MitoTracker but co-localized with caveolin-3 in the sarcolemma of mouse gastrocnemius. Mitochondrial matrix-facing OXPHOS complex subunits were ectopically expressed in the sarcolemma of the non-permeabilized muscle fibers and C2C12 myotubes. The sarcolemmal localization of cytochrome c was also observed from mouse gastrocnemius muscles and C2C12 myotubes, as determined by confocal and total internal resonance fluorescence (TIRF) microscopy. Based on these data, we conclude that a portion of OXPHOS complexes is localized in the sarcolemma of skeletal muscle and may have non-canonical functions.

Original languageEnglish
Pages (from-to)116-121
Number of pages6
JournalBMB Reports
Volume49
Issue number2
DOIs
Publication statusPublished - 2016

Keywords

  • Cytochrome c
  • Extracellular NADH-dependent respiration
  • Oxidative phosphorylation complexes
  • Sarcolemma
  • Skeletal muscle

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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