Mitogen activated protein kinase pathway-dependent effects of platelet-derived growth factor on migration of trophectoderm cells

Wooyoung Jeong, Gwonhwa Song, Jinyoung Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Successful development of the conceptus and implantation requires an intimate trophic connection between maternal uterus and conceptus mediated by local regulators including growth factors. Platelet-derived growth factor (PDGF) acts as a chemotactic factor for a variety of cell types. Current studies have determined that PDGF participates in rapid growth and development of cleavage stage embryos, but PDGF-induced effects on the growth and development of peri-implantation conceptus remains unknown. In the present study, PDGF induced phosphorylation of ERK1/2, AKT and RPS6 proteins in porcine trophectoderm (pTr) cells in a dose- and time-dependent manner. Addition of U0126 (an inhibitor of ERK1/2) or LY294002 (a PI3K inhibitor) blocked PDGF-induced effects on phosphorylation of signaling proteins. Combinations of PDGF and U0126 decreased PDGF-induced p-ERK1/2 and p-AKT1, but combinations of PDGF and LY294002 blocked only PDGF-induced AKT phosphorylation. Furthermore, PDGF significantly induced pTr cell migration and these stimulatory effects were blocked by U0126 and LY294002. Immunoreactive p-ERK1/2 and p-RPS6 proteins were abundant in pTr cells treated with PDGF, but U0126 reduced PDGF-induced p-ERK1/2 and p-RPS6 levels to basal amounts. Present study suggests that PDGF secreted into the maternal-conceptus microenvironment stimulates pTr cell migration through signal transduction cascades mediated by the ERK1/2 MAPK and AKT1 pathways.

Original languageEnglish
Pages (from-to)575-581
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume463
Issue number4
DOIs
Publication statusPublished - 2015 May 22

Fingerprint

Platelet-Derived Growth Factor
Mitogen-Activated Protein Kinases
Cell Movement
Phosphorylation
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Swine
Growth and Development
Mothers
Signal transduction
Proteins
Chemotactic Factors
Phosphatidylinositol 3-Kinases
Uterus
Signal Transduction
Intercellular Signaling Peptides and Proteins
Embryonic Structures

Keywords

  • Migration
  • PDGF
  • Peri-implantation
  • Trophectoderm

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Mitogen activated protein kinase pathway-dependent effects of platelet-derived growth factor on migration of trophectoderm cells. / Jeong, Wooyoung; Song, Gwonhwa; Kim, Jinyoung.

In: Biochemical and Biophysical Research Communications, Vol. 463, No. 4, 22.05.2015, p. 575-581.

Research output: Contribution to journalArticle

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