Mitomycin-C concentration in cornea and aqueous humor and apoptosis in the stroma after topical mitomycin-C application

Effects of mitomycin-C application time and concentration

Jong-Suk Song, Jun Heon Kim, Minho Yang, Dong Geun Sul, Hyo Myung Kim

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

PURPOSE: To evaluate the effects of the applied mitomycin-C (MMC) concentration and application time on the aqueous MMC concentration and apoptosis in the corneal stroma. METHODS: New Zealand white rabbits underwent mechanical epithelium debridement of the central 7.5 mm of the cornea. A sponge soaked in MMC solution was placed on the denuded corneal stroma. The effect of the exposure times ranging from 15 to 120 seconds and the different MMC concentrations ranging from 0.005% to 0.04% on the aqueous MMC concentration and the apoptosis in the stromal cells were evaluated. RESULTS: The aqueous concentration of MMC increased linearly with increasing exposure time and MMC concentration. The correlation between the aqueous MMC concentration and the applied concentration (r = 0.809, P < 0.001) was higher than the correlation between the aqueous MMC concentration and the application time (r = 0.693, P < 0.001). Terminal deoxyribonucleotidyltransferase-mediated dUTP-digoxigenin nick end labeling (TUNEL)-positive cells were detected in the superficial stroma of the central denuded cornea. The numbers of TUNEL-positive cells increased linearly with increasing concentrations, and the increase was statistically significant (P = 0.026). However, the numbers of TUNEL-positive cells increased only slightly with an increasing application time, and the increase was not statistically significant (P = 0.928). CONCLUSIONS: Reducing the applied concentration or decreasing the exposure time might be a good modality for reducing the potential MMC toxicity. The applied MMC concentration had greater effects on the aqueous MMC concentration and apoptosis in the stromal cells than the exposure time.

Original languageEnglish
Pages (from-to)461-467
Number of pages7
JournalCornea
Volume26
Issue number4
DOIs
Publication statusPublished - 2007 May 1

Fingerprint

Aqueous Humor
Mitomycin
Cornea
Apoptosis
In Situ Nick-End Labeling
Corneal Stroma
Stromal Cells
Digoxigenin
DNA Nucleotidylexotransferase
Porifera
Debridement
Epithelium

Keywords

  • Apoptosis
  • Aqueous concentration
  • High-performance liquid chromatography
  • Mitomycin C
  • Terminal deoxyribonucleotidyltransferase-mediated dUTP-digoxigenin nick end labeling stain

ASJC Scopus subject areas

  • Ophthalmology

Cite this

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title = "Mitomycin-C concentration in cornea and aqueous humor and apoptosis in the stroma after topical mitomycin-C application: Effects of mitomycin-C application time and concentration",
abstract = "PURPOSE: To evaluate the effects of the applied mitomycin-C (MMC) concentration and application time on the aqueous MMC concentration and apoptosis in the corneal stroma. METHODS: New Zealand white rabbits underwent mechanical epithelium debridement of the central 7.5 mm of the cornea. A sponge soaked in MMC solution was placed on the denuded corneal stroma. The effect of the exposure times ranging from 15 to 120 seconds and the different MMC concentrations ranging from 0.005{\%} to 0.04{\%} on the aqueous MMC concentration and the apoptosis in the stromal cells were evaluated. RESULTS: The aqueous concentration of MMC increased linearly with increasing exposure time and MMC concentration. The correlation between the aqueous MMC concentration and the applied concentration (r = 0.809, P < 0.001) was higher than the correlation between the aqueous MMC concentration and the application time (r = 0.693, P < 0.001). Terminal deoxyribonucleotidyltransferase-mediated dUTP-digoxigenin nick end labeling (TUNEL)-positive cells were detected in the superficial stroma of the central denuded cornea. The numbers of TUNEL-positive cells increased linearly with increasing concentrations, and the increase was statistically significant (P = 0.026). However, the numbers of TUNEL-positive cells increased only slightly with an increasing application time, and the increase was not statistically significant (P = 0.928). CONCLUSIONS: Reducing the applied concentration or decreasing the exposure time might be a good modality for reducing the potential MMC toxicity. The applied MMC concentration had greater effects on the aqueous MMC concentration and apoptosis in the stromal cells than the exposure time.",
keywords = "Apoptosis, Aqueous concentration, High-performance liquid chromatography, Mitomycin C, Terminal deoxyribonucleotidyltransferase-mediated dUTP-digoxigenin nick end labeling stain",
author = "Jong-Suk Song and Kim, {Jun Heon} and Minho Yang and Sul, {Dong Geun} and Kim, {Hyo Myung}",
year = "2007",
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TY - JOUR

T1 - Mitomycin-C concentration in cornea and aqueous humor and apoptosis in the stroma after topical mitomycin-C application

T2 - Effects of mitomycin-C application time and concentration

AU - Song, Jong-Suk

AU - Kim, Jun Heon

AU - Yang, Minho

AU - Sul, Dong Geun

AU - Kim, Hyo Myung

PY - 2007/5/1

Y1 - 2007/5/1

N2 - PURPOSE: To evaluate the effects of the applied mitomycin-C (MMC) concentration and application time on the aqueous MMC concentration and apoptosis in the corneal stroma. METHODS: New Zealand white rabbits underwent mechanical epithelium debridement of the central 7.5 mm of the cornea. A sponge soaked in MMC solution was placed on the denuded corneal stroma. The effect of the exposure times ranging from 15 to 120 seconds and the different MMC concentrations ranging from 0.005% to 0.04% on the aqueous MMC concentration and the apoptosis in the stromal cells were evaluated. RESULTS: The aqueous concentration of MMC increased linearly with increasing exposure time and MMC concentration. The correlation between the aqueous MMC concentration and the applied concentration (r = 0.809, P < 0.001) was higher than the correlation between the aqueous MMC concentration and the application time (r = 0.693, P < 0.001). Terminal deoxyribonucleotidyltransferase-mediated dUTP-digoxigenin nick end labeling (TUNEL)-positive cells were detected in the superficial stroma of the central denuded cornea. The numbers of TUNEL-positive cells increased linearly with increasing concentrations, and the increase was statistically significant (P = 0.026). However, the numbers of TUNEL-positive cells increased only slightly with an increasing application time, and the increase was not statistically significant (P = 0.928). CONCLUSIONS: Reducing the applied concentration or decreasing the exposure time might be a good modality for reducing the potential MMC toxicity. The applied MMC concentration had greater effects on the aqueous MMC concentration and apoptosis in the stromal cells than the exposure time.

AB - PURPOSE: To evaluate the effects of the applied mitomycin-C (MMC) concentration and application time on the aqueous MMC concentration and apoptosis in the corneal stroma. METHODS: New Zealand white rabbits underwent mechanical epithelium debridement of the central 7.5 mm of the cornea. A sponge soaked in MMC solution was placed on the denuded corneal stroma. The effect of the exposure times ranging from 15 to 120 seconds and the different MMC concentrations ranging from 0.005% to 0.04% on the aqueous MMC concentration and the apoptosis in the stromal cells were evaluated. RESULTS: The aqueous concentration of MMC increased linearly with increasing exposure time and MMC concentration. The correlation between the aqueous MMC concentration and the applied concentration (r = 0.809, P < 0.001) was higher than the correlation between the aqueous MMC concentration and the application time (r = 0.693, P < 0.001). Terminal deoxyribonucleotidyltransferase-mediated dUTP-digoxigenin nick end labeling (TUNEL)-positive cells were detected in the superficial stroma of the central denuded cornea. The numbers of TUNEL-positive cells increased linearly with increasing concentrations, and the increase was statistically significant (P = 0.026). However, the numbers of TUNEL-positive cells increased only slightly with an increasing application time, and the increase was not statistically significant (P = 0.928). CONCLUSIONS: Reducing the applied concentration or decreasing the exposure time might be a good modality for reducing the potential MMC toxicity. The applied MMC concentration had greater effects on the aqueous MMC concentration and apoptosis in the stromal cells than the exposure time.

KW - Apoptosis

KW - Aqueous concentration

KW - High-performance liquid chromatography

KW - Mitomycin C

KW - Terminal deoxyribonucleotidyltransferase-mediated dUTP-digoxigenin nick end labeling stain

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U2 - 10.1097/ICO.0b013e318030d217

DO - 10.1097/ICO.0b013e318030d217

M3 - Article

VL - 26

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EP - 467

JO - Cornea

JF - Cornea

SN - 0277-3740

IS - 4

ER -