Mixotrophic growth with acetate or volatile fatty acids maximizes growth and lipid production in Chlamydomonas reinhardtii

Myounghoon Moon, Chul Woong Kim, Won Kun Park, Gursong Yoo, Yoon-E Choi, Ji Won Yang

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

We measured the growth of and lipid production by the model microalga Chlamydomonas reinhardtii under different phototrophic, heterotrophic, and mixotrophic conditions to determine the optimal conditions for growth and biodiesel production. In particular, we examined cell growth and yield of fatty acid methyl esters (FAMEs) when C. reinhardtii was cultured in the presence of different organic carbon sources (acetate, glucose, glycerol, and sucrose). C. reinhardtii grew under various conditions, but mixotrophic cultivation was best. The greatest biomass production (2.15gL-1 in 5days) and FAME yield (16.41% of biomass) were observed under mixotrophic cultivation with acetate (10gL-1). As an alternative to acetate, we additionally tested the use of volatile fatty acids (VFAs; acetic, propionic, and butyric acids), which can be inexpensively produced through fermentation of food waste. The highest FAME yield (19.02% of biomass) and biomass production (2.05gL-1 in 5days) were obtained with 5gL-1 of VFAs. This result indicates that VFAs can serve as an inexpensive alternative carbon source for maximizing lipid production in mixotrophic cultivation of C. reinhardtii.

Original languageEnglish
Pages (from-to)352-357
Number of pages6
JournalAlgal Research
Volume2
Issue number4
DOIs
Publication statusPublished - 2013 Oct 1
Externally publishedYes

Fingerprint

Chlamydomonas reinhardtii
volatile fatty acids
acetates
lipids
biomass production
fermentation wastes
food waste
carbon
biomass
butyric acid
propionic acid
biodiesel
glycerol
cell growth
sucrose
glucose
fatty acid methyl esters

Keywords

  • Acetate
  • Chlamydomonas reinhardtii
  • Fatty acid methyl ester (FAME)
  • Microalgae
  • Mixotrophic cultivation
  • Volatile fatty acids (VFAs)

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Mixotrophic growth with acetate or volatile fatty acids maximizes growth and lipid production in Chlamydomonas reinhardtii. / Moon, Myounghoon; Kim, Chul Woong; Park, Won Kun; Yoo, Gursong; Choi, Yoon-E; Yang, Ji Won.

In: Algal Research, Vol. 2, No. 4, 01.10.2013, p. 352-357.

Research output: Contribution to journalArticle

Moon, Myounghoon ; Kim, Chul Woong ; Park, Won Kun ; Yoo, Gursong ; Choi, Yoon-E ; Yang, Ji Won. / Mixotrophic growth with acetate or volatile fatty acids maximizes growth and lipid production in Chlamydomonas reinhardtii. In: Algal Research. 2013 ; Vol. 2, No. 4. pp. 352-357.
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