Abstract
3,6-Anhydro-l-galactose (l-AHG) is a bioactive constituent of agar polysaccharides. To be used as a cosmetic or pharmaceutical ingredient, l-AHG is more favorably prepared by enzymatic saccharification of agar using a combination of agarolytic enzymes. Determining the optimum enzyme combination from the natural repertoire is a bottleneck for designing an efficient enzymatic-hydrolysis process. We consider all theoretical enzymatic-saccharification routes in the natural agarolytic pathway of a marine bacterium, Saccharophagus degradans 2-40. Among these routes, three representative routes were determined by removing redundant enzymatic reactions. We simulated each l-AHG production route with simple kinetic models and validated the reaction feasibility with an experimental procedure. The optimal enzyme mixture (with 67.3% maximum saccharification yield) was composed of endotype β-agarase, exotype β-agarase, agarooligosaccharolytic β-galactosidase, and α-neoagarobiose hydrolase. This approach will reduce the time and effort needed for developing a coherent enzymatic process to produce l-AHG on a mass scale.
| Original language | English |
|---|---|
| Pages (from-to) | 6814-6821 |
| Number of pages | 8 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 66 |
| Issue number | 26 |
| DOIs | |
| Publication status | Published - 2018 Jul 5 |
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Keywords
- 3,6-anhydro- l -galactose
- agar
- agarase
- agarolytic pathway
- empirical model
- enzymatic saccharification
- red algal biomass
ASJC Scopus subject areas
- Chemistry(all)
- Agricultural and Biological Sciences(all)
Cite this
Model-Based Complete Enzymatic Production of 3,6-Anhydro- l -galactose from Red Algal Biomass. / Pathiraja, Duleepa; Lee, Saeyoung; Choi, In-Geol.
In: Journal of Agricultural and Food Chemistry, Vol. 66, No. 26, 05.07.2018, p. 6814-6821.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Model-Based Complete Enzymatic Production of 3,6-Anhydro- l -galactose from Red Algal Biomass
AU - Pathiraja, Duleepa
AU - Lee, Saeyoung
AU - Choi, In-Geol
PY - 2018/7/5
Y1 - 2018/7/5
N2 - 3,6-Anhydro-l-galactose (l-AHG) is a bioactive constituent of agar polysaccharides. To be used as a cosmetic or pharmaceutical ingredient, l-AHG is more favorably prepared by enzymatic saccharification of agar using a combination of agarolytic enzymes. Determining the optimum enzyme combination from the natural repertoire is a bottleneck for designing an efficient enzymatic-hydrolysis process. We consider all theoretical enzymatic-saccharification routes in the natural agarolytic pathway of a marine bacterium, Saccharophagus degradans 2-40. Among these routes, three representative routes were determined by removing redundant enzymatic reactions. We simulated each l-AHG production route with simple kinetic models and validated the reaction feasibility with an experimental procedure. The optimal enzyme mixture (with 67.3% maximum saccharification yield) was composed of endotype β-agarase, exotype β-agarase, agarooligosaccharolytic β-galactosidase, and α-neoagarobiose hydrolase. This approach will reduce the time and effort needed for developing a coherent enzymatic process to produce l-AHG on a mass scale.
AB - 3,6-Anhydro-l-galactose (l-AHG) is a bioactive constituent of agar polysaccharides. To be used as a cosmetic or pharmaceutical ingredient, l-AHG is more favorably prepared by enzymatic saccharification of agar using a combination of agarolytic enzymes. Determining the optimum enzyme combination from the natural repertoire is a bottleneck for designing an efficient enzymatic-hydrolysis process. We consider all theoretical enzymatic-saccharification routes in the natural agarolytic pathway of a marine bacterium, Saccharophagus degradans 2-40. Among these routes, three representative routes were determined by removing redundant enzymatic reactions. We simulated each l-AHG production route with simple kinetic models and validated the reaction feasibility with an experimental procedure. The optimal enzyme mixture (with 67.3% maximum saccharification yield) was composed of endotype β-agarase, exotype β-agarase, agarooligosaccharolytic β-galactosidase, and α-neoagarobiose hydrolase. This approach will reduce the time and effort needed for developing a coherent enzymatic process to produce l-AHG on a mass scale.
KW - 3,6-anhydro- l -galactose
KW - agar
KW - agarase
KW - agarolytic pathway
KW - empirical model
KW - enzymatic saccharification
KW - red algal biomass
UR - http://www.scopus.com/inward/record.url?scp=85048664580&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85048664580&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.8b01792
DO - 10.1021/acs.jafc.8b01792
M3 - Article
VL - 66
SP - 6814
EP - 6821
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 26
ER -