Model-Based Complete Enzymatic Production of 3,6-Anhydro- l -galactose from Red Algal Biomass

Duleepa Pathiraja, Saeyoung Lee, In-Geol Choi

Research output: Contribution to journalArticle

Abstract

3,6-Anhydro-l-galactose (l-AHG) is a bioactive constituent of agar polysaccharides. To be used as a cosmetic or pharmaceutical ingredient, l-AHG is more favorably prepared by enzymatic saccharification of agar using a combination of agarolytic enzymes. Determining the optimum enzyme combination from the natural repertoire is a bottleneck for designing an efficient enzymatic-hydrolysis process. We consider all theoretical enzymatic-saccharification routes in the natural agarolytic pathway of a marine bacterium, Saccharophagus degradans 2-40. Among these routes, three representative routes were determined by removing redundant enzymatic reactions. We simulated each l-AHG production route with simple kinetic models and validated the reaction feasibility with an experimental procedure. The optimal enzyme mixture (with 67.3% maximum saccharification yield) was composed of endotype β-agarase, exotype β-agarase, agarooligosaccharolytic β-galactosidase, and α-neoagarobiose hydrolase. This approach will reduce the time and effort needed for developing a coherent enzymatic process to produce l-AHG on a mass scale.

Original languageEnglish
Pages (from-to)6814-6821
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume66
Issue number26
DOIs
Publication statusPublished - 2018 Jul 5

Fingerprint

Saccharification
saccharification
Galactose
galactose
Biomass
Agar
biomass
Enzymes
enzymes
agar
Galactosidases
galactosidases
Enzymatic hydrolysis
Cosmetics
enzymatic reactions
cosmetics
enzymatic hydrolysis
hydrolases
Polysaccharides
Bacteria

Keywords

  • 3,6-anhydro- l -galactose
  • agar
  • agarase
  • agarolytic pathway
  • empirical model
  • enzymatic saccharification
  • red algal biomass

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Model-Based Complete Enzymatic Production of 3,6-Anhydro- l -galactose from Red Algal Biomass. / Pathiraja, Duleepa; Lee, Saeyoung; Choi, In-Geol.

In: Journal of Agricultural and Food Chemistry, Vol. 66, No. 26, 05.07.2018, p. 6814-6821.

Research output: Contribution to journalArticle

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