Modeling Rett Syndrome Using TALEN-Edited MECP2 Mutant Cynomolgus Monkeys

Yongchang Chen, Juehua Yu, Yuyu Niu, Dongdong Qin, Hailiang Liu, Gang Li, Yingzhou Hu, Jiaojian Wang, Yi Lu, Yu Kang, Yong Jiang, Kunhua Wu, Siguang Li, Jingkuan Wei, Jing He, Junbang Wang, Xiaojing Liu, Yuping Luo, Chenyang Si, Raoxian BaiKunshan Zhang, Jie Liu, Shaoyong Huang, Zhenzhen Chen, Shuang Wang, Xiaoying Chen, Xinhua Bao, Qingping Zhang, Fuxing Li, Rui Geng, Aibin Liang, Dinggang Shen, Tianzi Jiang, Xintian Hu, Yuanye Ma, Weizhi Ji, Yi Eve Sun

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Gene-editing technologies have made it feasible to create nonhuman primate models for human genetic disorders. Here, we report detailed genotypes and phenotypes of TALEN-edited MECP2 mutant cynomolgus monkeys serving as a model for a neurodevelopmental disorder, Rett syndrome (RTT), which is caused by loss-of-function mutations in the human MECP2 gene. Male mutant monkeys were embryonic lethal, reiterating that RTT is a disease of females. Through a battery of behavioral analyses, including primate-unique eye-tracking tests, in combination with brain imaging via MRI, we found a series of physiological, behavioral, and structural abnormalities resembling clinical manifestations of RTT. Moreover, blood transcriptome profiling revealed that mutant monkeys resembled RTT patients in immune gene dysregulation. Taken together, the stark similarity in phenotype and/or endophenotype between monkeys and patients suggested that gene-edited RTT founder monkeys would be of value for disease mechanistic studies as well as development of potential therapeutic interventions for RTT.

Original languageEnglish
Pages (from-to)945-955.e10
JournalCell
Volume169
Issue number5
DOIs
Publication statusPublished - 2017 May 18
Externally publishedYes

Fingerprint

Rett Syndrome
Macaca fascicularis
Genes
Haplorhini
Primates
Magnetic resonance imaging
Endophenotypes
Phenotype
Brain
Inborn Genetic Diseases
Blood
Medical Genetics
Gene Expression Profiling
Imaging techniques
Neuroimaging
Transcription Activator-Like Effector Nucleases
Genotype
Technology
Mutation

Keywords

  • disease models
  • gene editing
  • male embryonic lethal
  • MECP2
  • neurodevelopmental disorder
  • non-human primate
  • Rett syndrome

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Chen, Y., Yu, J., Niu, Y., Qin, D., Liu, H., Li, G., ... Sun, Y. E. (2017). Modeling Rett Syndrome Using TALEN-Edited MECP2 Mutant Cynomolgus Monkeys. Cell, 169(5), 945-955.e10. https://doi.org/10.1016/j.cell.2017.04.035

Modeling Rett Syndrome Using TALEN-Edited MECP2 Mutant Cynomolgus Monkeys. / Chen, Yongchang; Yu, Juehua; Niu, Yuyu; Qin, Dongdong; Liu, Hailiang; Li, Gang; Hu, Yingzhou; Wang, Jiaojian; Lu, Yi; Kang, Yu; Jiang, Yong; Wu, Kunhua; Li, Siguang; Wei, Jingkuan; He, Jing; Wang, Junbang; Liu, Xiaojing; Luo, Yuping; Si, Chenyang; Bai, Raoxian; Zhang, Kunshan; Liu, Jie; Huang, Shaoyong; Chen, Zhenzhen; Wang, Shuang; Chen, Xiaoying; Bao, Xinhua; Zhang, Qingping; Li, Fuxing; Geng, Rui; Liang, Aibin; Shen, Dinggang; Jiang, Tianzi; Hu, Xintian; Ma, Yuanye; Ji, Weizhi; Sun, Yi Eve.

In: Cell, Vol. 169, No. 5, 18.05.2017, p. 945-955.e10.

Research output: Contribution to journalArticle

Chen, Y, Yu, J, Niu, Y, Qin, D, Liu, H, Li, G, Hu, Y, Wang, J, Lu, Y, Kang, Y, Jiang, Y, Wu, K, Li, S, Wei, J, He, J, Wang, J, Liu, X, Luo, Y, Si, C, Bai, R, Zhang, K, Liu, J, Huang, S, Chen, Z, Wang, S, Chen, X, Bao, X, Zhang, Q, Li, F, Geng, R, Liang, A, Shen, D, Jiang, T, Hu, X, Ma, Y, Ji, W & Sun, YE 2017, 'Modeling Rett Syndrome Using TALEN-Edited MECP2 Mutant Cynomolgus Monkeys', Cell, vol. 169, no. 5, pp. 945-955.e10. https://doi.org/10.1016/j.cell.2017.04.035
Chen Y, Yu J, Niu Y, Qin D, Liu H, Li G et al. Modeling Rett Syndrome Using TALEN-Edited MECP2 Mutant Cynomolgus Monkeys. Cell. 2017 May 18;169(5):945-955.e10. https://doi.org/10.1016/j.cell.2017.04.035
Chen, Yongchang ; Yu, Juehua ; Niu, Yuyu ; Qin, Dongdong ; Liu, Hailiang ; Li, Gang ; Hu, Yingzhou ; Wang, Jiaojian ; Lu, Yi ; Kang, Yu ; Jiang, Yong ; Wu, Kunhua ; Li, Siguang ; Wei, Jingkuan ; He, Jing ; Wang, Junbang ; Liu, Xiaojing ; Luo, Yuping ; Si, Chenyang ; Bai, Raoxian ; Zhang, Kunshan ; Liu, Jie ; Huang, Shaoyong ; Chen, Zhenzhen ; Wang, Shuang ; Chen, Xiaoying ; Bao, Xinhua ; Zhang, Qingping ; Li, Fuxing ; Geng, Rui ; Liang, Aibin ; Shen, Dinggang ; Jiang, Tianzi ; Hu, Xintian ; Ma, Yuanye ; Ji, Weizhi ; Sun, Yi Eve. / Modeling Rett Syndrome Using TALEN-Edited MECP2 Mutant Cynomolgus Monkeys. In: Cell. 2017 ; Vol. 169, No. 5. pp. 945-955.e10.
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