Modeling the inactivation of Escherichia coli O157

H7 and generic Escherichia coli by supercritical carbon dioxide

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45 Citations (Scopus)

Abstract

In this study, supercritical carbon dioxide (SC-CO2) was applied in the inactivation of pathogenic Escherichia coli (E. coli) O157:H7 and generic E. coli. For both strains suspended in physiological saline (PS), colony forming units per ml were reduced by 8 log orders within 15-30 min, in a treatment range of 80-150 bar and 35-45 °C. Any significant differences between the two E. coli strains during the inactivation by SC-CO2 were not noticed. The microbial inactivation curve, which was established by the modified Gompertz model describing the survival rate with treatment time, was divided into three distinct stages. When using cells in PS, kdm, λ and t8 (the time for an 8-log reduction of cell counts) were 0 to 3 min- 1, 8 to 16 min and 11 to 29 min, respectively. The temperature-dependency of the microbial inactivation was verified via the correlation of the logarithm of kdm versus the inverse of temperature. We have observed lower inactivation rates in phosphate-buffered saline (PBS) than in PS, the lowered pH, and an increase of UV-absorbing substances in the cell suspension after SC-CO2 treatment. Also, the deformation and collapse of the SC-CO2-treated cells were revealed by scanning and transmission electron microscopy, and the deactivation of cellular enzymes occurred. These all suggest that the inactivation of E. coli O157:H7 and generic E. coli was possibly caused in a concerted manner by acidification, damage to cell membranes and subsequent leakage of cellular materials, and the inactivation of cellular enzymes.

Original languageEnglish
Pages (from-to)52-61
Number of pages10
JournalInternational Journal of Food Microbiology
Volume118
Issue number1
DOIs
Publication statusPublished - 2007 Aug 15

Fingerprint

Escherichia coli O157
Carbon Dioxide
Escherichia coli
Microbial Viability
Carbon dioxide
inactivation
carbon dioxide
Scanning Transmission Electron Microscopy
Temperature
Enzymes
Suspensions
Stem Cells
Cell Count
Phosphates
Cell Membrane
Acidification
Cell membranes
cells
Cells
enzymes

Keywords

  • Escherichia coli O157:H7
  • Inactivation of microorganisms
  • Sterilization
  • Supercritical CO

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

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title = "Modeling the inactivation of Escherichia coli O157: H7 and generic Escherichia coli by supercritical carbon dioxide",
abstract = "In this study, supercritical carbon dioxide (SC-CO2) was applied in the inactivation of pathogenic Escherichia coli (E. coli) O157:H7 and generic E. coli. For both strains suspended in physiological saline (PS), colony forming units per ml were reduced by 8 log orders within 15-30 min, in a treatment range of 80-150 bar and 35-45 °C. Any significant differences between the two E. coli strains during the inactivation by SC-CO2 were not noticed. The microbial inactivation curve, which was established by the modified Gompertz model describing the survival rate with treatment time, was divided into three distinct stages. When using cells in PS, kdm, λ and t8 (the time for an 8-log reduction of cell counts) were 0 to 3 min- 1, 8 to 16 min and 11 to 29 min, respectively. The temperature-dependency of the microbial inactivation was verified via the correlation of the logarithm of kdm versus the inverse of temperature. We have observed lower inactivation rates in phosphate-buffered saline (PBS) than in PS, the lowered pH, and an increase of UV-absorbing substances in the cell suspension after SC-CO2 treatment. Also, the deformation and collapse of the SC-CO2-treated cells were revealed by scanning and transmission electron microscopy, and the deactivation of cellular enzymes occurred. These all suggest that the inactivation of E. coli O157:H7 and generic E. coli was possibly caused in a concerted manner by acidification, damage to cell membranes and subsequent leakage of cellular materials, and the inactivation of cellular enzymes.",
keywords = "Escherichia coli O157:H7, Inactivation of microorganisms, Sterilization, Supercritical CO",
author = "Kim, {Soo Rin} and Min-Suk Rhee and Byoung-Chul Kim and Kim, {Kyoung Heon}",
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T2 - H7 and generic Escherichia coli by supercritical carbon dioxide

AU - Kim, Soo Rin

AU - Rhee, Min-Suk

AU - Kim, Byoung-Chul

AU - Kim, Kyoung Heon

PY - 2007/8/15

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N2 - In this study, supercritical carbon dioxide (SC-CO2) was applied in the inactivation of pathogenic Escherichia coli (E. coli) O157:H7 and generic E. coli. For both strains suspended in physiological saline (PS), colony forming units per ml were reduced by 8 log orders within 15-30 min, in a treatment range of 80-150 bar and 35-45 °C. Any significant differences between the two E. coli strains during the inactivation by SC-CO2 were not noticed. The microbial inactivation curve, which was established by the modified Gompertz model describing the survival rate with treatment time, was divided into three distinct stages. When using cells in PS, kdm, λ and t8 (the time for an 8-log reduction of cell counts) were 0 to 3 min- 1, 8 to 16 min and 11 to 29 min, respectively. The temperature-dependency of the microbial inactivation was verified via the correlation of the logarithm of kdm versus the inverse of temperature. We have observed lower inactivation rates in phosphate-buffered saline (PBS) than in PS, the lowered pH, and an increase of UV-absorbing substances in the cell suspension after SC-CO2 treatment. Also, the deformation and collapse of the SC-CO2-treated cells were revealed by scanning and transmission electron microscopy, and the deactivation of cellular enzymes occurred. These all suggest that the inactivation of E. coli O157:H7 and generic E. coli was possibly caused in a concerted manner by acidification, damage to cell membranes and subsequent leakage of cellular materials, and the inactivation of cellular enzymes.

AB - In this study, supercritical carbon dioxide (SC-CO2) was applied in the inactivation of pathogenic Escherichia coli (E. coli) O157:H7 and generic E. coli. For both strains suspended in physiological saline (PS), colony forming units per ml were reduced by 8 log orders within 15-30 min, in a treatment range of 80-150 bar and 35-45 °C. Any significant differences between the two E. coli strains during the inactivation by SC-CO2 were not noticed. The microbial inactivation curve, which was established by the modified Gompertz model describing the survival rate with treatment time, was divided into three distinct stages. When using cells in PS, kdm, λ and t8 (the time for an 8-log reduction of cell counts) were 0 to 3 min- 1, 8 to 16 min and 11 to 29 min, respectively. The temperature-dependency of the microbial inactivation was verified via the correlation of the logarithm of kdm versus the inverse of temperature. We have observed lower inactivation rates in phosphate-buffered saline (PBS) than in PS, the lowered pH, and an increase of UV-absorbing substances in the cell suspension after SC-CO2 treatment. Also, the deformation and collapse of the SC-CO2-treated cells were revealed by scanning and transmission electron microscopy, and the deactivation of cellular enzymes occurred. These all suggest that the inactivation of E. coli O157:H7 and generic E. coli was possibly caused in a concerted manner by acidification, damage to cell membranes and subsequent leakage of cellular materials, and the inactivation of cellular enzymes.

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