Modification of professional antigen-presenting cells with small interfering RNA in vivo to enhance cancer vaccine potency

Tae Woo Kim, Jin Hyup Lee, Liangmei He, David A K Boyd, J. Marie Hardwick, Chien Fu Hung, T. C. Wu

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

RNA interference using small interfering RNA (siRNA) is an effective means of silencing gene expression in cells. Intradermal administration of nucleic acids via gene gun represents an efficient method for delivering nucleic acids to professional antigen-presenting cells in vivo. In this study, we show that the coadministration of DNA vaccines encoding human papillomavirus type 16 E7 with siRNA targeting key proapoptotic proteins Bak and Bax prolongs the lives of antigen-expressing dendritic cells in the draining lymph nodes, enhances antigen-specific CD8+ T-cell responses, and elicits potent antitumor effects against an E7-expressing tumor model in vaccinated mice. Our data indicate that intradermal administration of siRNA to manipulate gene expression represents a plausible strategy for modification of the properties of professional antigen-presenting cells in vivo to enhance cancer vaccine potency.

Original languageEnglish
Pages (from-to)309-316
Number of pages8
JournalCancer Research
Volume65
Issue number1
Publication statusPublished - 2005 Jan 1
Externally publishedYes

Fingerprint

Vaccine Potency
Cancer Vaccines
Antigen-Presenting Cells
Small Interfering RNA
Nucleic Acids
bcl-2 Homologous Antagonist-Killer Protein
CD8 Antigens
Gene Expression
bcl-2-Associated X Protein
DNA Vaccines
Human papillomavirus 16
Firearms
RNA Interference
Dendritic Cells
Lymph Nodes
T-Lymphocytes
Antigens
Genes
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kim, T. W., Lee, J. H., He, L., Boyd, D. A. K., Hardwick, J. M., Hung, C. F., & Wu, T. C. (2005). Modification of professional antigen-presenting cells with small interfering RNA in vivo to enhance cancer vaccine potency. Cancer Research, 65(1), 309-316.

Modification of professional antigen-presenting cells with small interfering RNA in vivo to enhance cancer vaccine potency. / Kim, Tae Woo; Lee, Jin Hyup; He, Liangmei; Boyd, David A K; Hardwick, J. Marie; Hung, Chien Fu; Wu, T. C.

In: Cancer Research, Vol. 65, No. 1, 01.01.2005, p. 309-316.

Research output: Contribution to journalArticle

Kim, TW, Lee, JH, He, L, Boyd, DAK, Hardwick, JM, Hung, CF & Wu, TC 2005, 'Modification of professional antigen-presenting cells with small interfering RNA in vivo to enhance cancer vaccine potency', Cancer Research, vol. 65, no. 1, pp. 309-316.
Kim, Tae Woo ; Lee, Jin Hyup ; He, Liangmei ; Boyd, David A K ; Hardwick, J. Marie ; Hung, Chien Fu ; Wu, T. C. / Modification of professional antigen-presenting cells with small interfering RNA in vivo to enhance cancer vaccine potency. In: Cancer Research. 2005 ; Vol. 65, No. 1. pp. 309-316.
@article{4f977f119bd740f2b515c6ed630c4887,
title = "Modification of professional antigen-presenting cells with small interfering RNA in vivo to enhance cancer vaccine potency",
abstract = "RNA interference using small interfering RNA (siRNA) is an effective means of silencing gene expression in cells. Intradermal administration of nucleic acids via gene gun represents an efficient method for delivering nucleic acids to professional antigen-presenting cells in vivo. In this study, we show that the coadministration of DNA vaccines encoding human papillomavirus type 16 E7 with siRNA targeting key proapoptotic proteins Bak and Bax prolongs the lives of antigen-expressing dendritic cells in the draining lymph nodes, enhances antigen-specific CD8+ T-cell responses, and elicits potent antitumor effects against an E7-expressing tumor model in vaccinated mice. Our data indicate that intradermal administration of siRNA to manipulate gene expression represents a plausible strategy for modification of the properties of professional antigen-presenting cells in vivo to enhance cancer vaccine potency.",
author = "Kim, {Tae Woo} and Lee, {Jin Hyup} and Liangmei He and Boyd, {David A K} and Hardwick, {J. Marie} and Hung, {Chien Fu} and Wu, {T. C.}",
year = "2005",
month = "1",
day = "1",
language = "English",
volume = "65",
pages = "309--316",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "1",

}

TY - JOUR

T1 - Modification of professional antigen-presenting cells with small interfering RNA in vivo to enhance cancer vaccine potency

AU - Kim, Tae Woo

AU - Lee, Jin Hyup

AU - He, Liangmei

AU - Boyd, David A K

AU - Hardwick, J. Marie

AU - Hung, Chien Fu

AU - Wu, T. C.

PY - 2005/1/1

Y1 - 2005/1/1

N2 - RNA interference using small interfering RNA (siRNA) is an effective means of silencing gene expression in cells. Intradermal administration of nucleic acids via gene gun represents an efficient method for delivering nucleic acids to professional antigen-presenting cells in vivo. In this study, we show that the coadministration of DNA vaccines encoding human papillomavirus type 16 E7 with siRNA targeting key proapoptotic proteins Bak and Bax prolongs the lives of antigen-expressing dendritic cells in the draining lymph nodes, enhances antigen-specific CD8+ T-cell responses, and elicits potent antitumor effects against an E7-expressing tumor model in vaccinated mice. Our data indicate that intradermal administration of siRNA to manipulate gene expression represents a plausible strategy for modification of the properties of professional antigen-presenting cells in vivo to enhance cancer vaccine potency.

AB - RNA interference using small interfering RNA (siRNA) is an effective means of silencing gene expression in cells. Intradermal administration of nucleic acids via gene gun represents an efficient method for delivering nucleic acids to professional antigen-presenting cells in vivo. In this study, we show that the coadministration of DNA vaccines encoding human papillomavirus type 16 E7 with siRNA targeting key proapoptotic proteins Bak and Bax prolongs the lives of antigen-expressing dendritic cells in the draining lymph nodes, enhances antigen-specific CD8+ T-cell responses, and elicits potent antitumor effects against an E7-expressing tumor model in vaccinated mice. Our data indicate that intradermal administration of siRNA to manipulate gene expression represents a plausible strategy for modification of the properties of professional antigen-presenting cells in vivo to enhance cancer vaccine potency.

UR - http://www.scopus.com/inward/record.url?scp=11244319876&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11244319876&partnerID=8YFLogxK

M3 - Article

VL - 65

SP - 309

EP - 316

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 1

ER -