Modified acellularization for successful vascular xenotransplantation

Won Min Jo, Young Sang Sohn, Ho Choi Young, Jei Kim Hark, Deuk Cho Hyun

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The purpose of this study was to estimate the possibilities of an acellular matrix using a modified acellularization protocol, which circumvents immunological, microbiological, and physiological barriers. We treated porcine subclavian arteries with various reagents to construct acellular grafts. Afterwards, these grafts were interposed in a mongrel dogs' abdominal aorta. Six dogs underwent interposition with fresh porcine grafts (control group), and seven had interposed acellular grafts (acellular group). The control and acellular group dogs were sacrificed at 1, 3, 5 (n=2 in each group) and 12 months (n=1 in acellular group) after the operation. Histopathological examinations were then performed, to assess the degree to which re-endothelialization, inflammation, thrombus formation, and calcification occurred. The entire acellular group, but none of the control group, exhibited re-endothelialization. The degrees to which inflammation, thrombosis, and calcification occurred were found to be lower in the acellular group. We also discovered many smooth muscle cells in the medial layer of the xenograft that had been implanted in the dog sacrificed 12 months after the operation. These results suggest that the construction of xenografts using our modified acellularization protocol may offer acceptable outcomes as a vascular xenograft.

Original languageEnglish
Pages (from-to)262-269
Number of pages8
JournalJournal of Korean medical science
Volume22
Issue number2
DOIs
Publication statusPublished - 2007 Apr

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Keywords

  • Graft rejection
  • Vascular endothelial cell
  • Xenograft

ASJC Scopus subject areas

  • Medicine(all)

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