Modified suppression subtractive hybridization identifies an AP2-containing protein involved in metal responses in physcomitrella patens

Sung Hyun Cho, Quoc Truong Hoang, Jeong Won Phee, Yun Young Kim, Hyun Young Shin, Jeong Sheop Shin

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The moss Physcomitrella patens has two life cycles, filamentous protonema and leafy gametophore. A modified from of suppression subtractive hybridization (SSH), mirror orientation selection (MOS), was applied to screen genes differentially expressed in the P. patens protonema. Using reverse Northern blot analysis, differentially expressed clones were identified. The identified genes were involved mainly in metal binding and detoxification. One of these genes was an AP2 (APETALA2) domain-containing protein (PpACP1), which was highly up-regulated in the protonema. Alignment with other AP2/EREBPs (Ethylene Responsive Element Binding Proteins) revealed significant sequence homology of the deduced amino acid sequence in the AP2/EREBP DNA binding domain. Northern analysis under various stress conditions showed that PpACP1 was induced by ethephon, cadmium, copper, ABA, IAA, and cold. In addition, it was highly expressed in suspension-cultured protonema. We suggest that PpACP1 is involved in responses to metals, and that suspension culture enhance the expression of genes responding to metals.

Original languageEnglish
Pages (from-to)100-107
Number of pages8
JournalMolecules and cells
Volume23
Issue number1
Publication statusPublished - 2007 Feb

Keywords

  • AP2-containing protein
  • Physcomitrella patens
  • Protonema
  • Stress-induced
  • Suppression subtractive hybridization
  • Suspension culture

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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