Modified vacuole phenotype1 is an arabidopsis myrosinase-associated protein involved in endomembrane protein trafficking

April E. Agee, Marci Surpin, Eun J. Sohn, Thomas Girke, Abel Rosado, Brian W. Kram, Clay Carter, Adam M. Wentzell, Daniel J. Kliebenstein, Hak Chul Jin, Ohkmae Kim, Hailing Jin, Glenn R. Hicks, Natasha V. Raikhel

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Abstract

We identified an Arabidopsis (Arabidopsis thaliana) ethyl methanesulfonate mutant, modified vacuole phenotype1-1 (mvp1-1), in a fluorescent confocal microscopy screen for plants with mislocalization of a green fluorescent protein-δ tonoplast intrinsic protein fusion. The mvp1-1 mutant displayed static perinuclear aggregates of the reporter protein. mvp1 mutants also exhibited a number of vacuole-related phenotypes, as demonstrated by defects in growth, utilization of stored carbon, gravitropic response, salt sensitivity, and specific susceptibility to the fungal necrotroph Alternaria brassicicola. Similarly, crosses with other endomembrane marker fusions identified mislocalization to aggregate structures, indicating a general defect in protein trafficking. Map-based cloning showed that the mvp1-1 mutation altered a gene encoding a putative myrosinase-associated protein, and glutathione S-transferase pull-down assays demonstrated that MVP1 interacted specifically with the Arabidopsis myrosinase protein, THIOGLUCOSIDE GLUCOHYDROLASE2 (TGG2), but not TGG1. Moreover, the mvp1-1 mutant showed increased nitrile production during glucosinolate hydrolysis, suggesting that MVP1 may play a role in modulation of myrosinase activity. We propose that MVP1 is a myrosinase-associated protein that functions, in part, to correctly localize the myrosinase TGG2 and prevent inappropriate glucosinolate hydrolysis that could generate cytotoxic molecules.

Original languageEnglish
Pages (from-to)120-132
Number of pages13
JournalPlant Physiology
Volume152
Issue number1
DOIs
Publication statusPublished - 2010 Jan 1

Fingerprint

thioglucosidase
protein transport
Protein Transport
Vacuoles
Arabidopsis
vacuoles
Thioglucosides
Glucosinolates
mutants
protein aggregates
glucosinolates
Proteins
proteins
Hydrolysis
hydrolysis
Arabidopsis Proteins
Alternaria brassicicola
Ethyl Methanesulfonate
nitriles
ethyl methanesulfonate

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Agee, A. E., Surpin, M., Sohn, E. J., Girke, T., Rosado, A., Kram, B. W., ... Raikhel, N. V. (2010). Modified vacuole phenotype1 is an arabidopsis myrosinase-associated protein involved in endomembrane protein trafficking. Plant Physiology, 152(1), 120-132. https://doi.org/10.1104/pp.109.145078

Modified vacuole phenotype1 is an arabidopsis myrosinase-associated protein involved in endomembrane protein trafficking. / Agee, April E.; Surpin, Marci; Sohn, Eun J.; Girke, Thomas; Rosado, Abel; Kram, Brian W.; Carter, Clay; Wentzell, Adam M.; Kliebenstein, Daniel J.; Jin, Hak Chul; Kim, Ohkmae; Jin, Hailing; Hicks, Glenn R.; Raikhel, Natasha V.

In: Plant Physiology, Vol. 152, No. 1, 01.01.2010, p. 120-132.

Research output: Contribution to journalArticle

Agee, AE, Surpin, M, Sohn, EJ, Girke, T, Rosado, A, Kram, BW, Carter, C, Wentzell, AM, Kliebenstein, DJ, Jin, HC, Kim, O, Jin, H, Hicks, GR & Raikhel, NV 2010, 'Modified vacuole phenotype1 is an arabidopsis myrosinase-associated protein involved in endomembrane protein trafficking', Plant Physiology, vol. 152, no. 1, pp. 120-132. https://doi.org/10.1104/pp.109.145078
Agee, April E. ; Surpin, Marci ; Sohn, Eun J. ; Girke, Thomas ; Rosado, Abel ; Kram, Brian W. ; Carter, Clay ; Wentzell, Adam M. ; Kliebenstein, Daniel J. ; Jin, Hak Chul ; Kim, Ohkmae ; Jin, Hailing ; Hicks, Glenn R. ; Raikhel, Natasha V. / Modified vacuole phenotype1 is an arabidopsis myrosinase-associated protein involved in endomembrane protein trafficking. In: Plant Physiology. 2010 ; Vol. 152, No. 1. pp. 120-132.
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