Modular laser-based endoluminal ablation of the gastrointestinal tract: in vivo dose–effect evaluation and predictive numerical model

Giuseppe Quero, Paola Saccomandi, Jung-Myun Kwak, Bernard Dallemagne, Guido Costamagna, Jacques Marescaux, Didier Mutter, Michele Diana

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Endoscopic submucosal dissection allows for “en bloc” removal of early gastrointestinal neoplasms. However, it is technically demanding and time-consuming. Alternatives could rely on energy-based techniques. We aimed to evaluate a predictive numerical model of thermal damage to preoperatively define optimal laser settings allowing for a controlled ablation down to the submucosa, and the ability of confocal endomicroscopy to provide damage information. Materials and methods: A Nd:YAG laser was applied onto the gastric mucosa of 21 Wistar rats on 10 spots (total 210). Power settings ranging from 0.5 to 2.5W were applied during 1–12 s, with a consequent energy delivery varying from 0.5 to 30 J. Out of the 210 samples, a total of 1050 hematoxilin–eosin stained slides were obtained. To evaluate thermal injury, the ratio between the damage depth (DD) over the mucosa and the submucosa thickness (T) was calculated. Effective and safe ablation was considered for a DD/T ratio ≤ 1 (only mucosal and submucosal damage). Confocal endomicroscopy was performed before and after ablation. A numerical model, using human physical properties, was developed to predict thermal damage. Results: No full-thickness perforations were detected. On histology, the DD/T ratio at 0.5 J was 0.57 ± 0.21, significantly lower when compared to energies ranging from 15 J (a DD/T ratio = 1.2 ± 0.3; p < 0.001) until 30 J (a DD/T ratio = 1.33 ± 0.31; p < 0.001). Safe mucosal and submucosal ablations were achieved applying energy between 4 and 12 J, never impairing the muscularis propria. Confocal endomicroscopy showed a distorted gland architecture. The predicted damage depth demonstrated a significant positive linear correlation with the experimental data (Pearson’s r 0.85; 95% CI 0.66–0.94). Conclusions: Low-power settings achieved effective and safe mucosal and submucosal ablation. The numerical model allowed for an accurate prediction of the ablated layers. Confocal endomicroscopy provided real-time thermal damage visualization. Further studies on larger animal models are required.

Original languageEnglish
JournalSurgical Endoscopy
DOIs
Publication statusAccepted/In press - 2018 Jan 1

Fingerprint

Gastrointestinal Tract
Lasers
Hot Temperature
Gastrointestinal Neoplasms
Solid-State Lasers
Gastric Mucosa
Wistar Rats
Histology
Mucous Membrane
Animal Models
Wounds and Injuries

Keywords

  • Confocal endomicroscopy
  • Early gastrointestinal cancer
  • Laser ablation
  • Preclinical study
  • Predictive numerical model

ASJC Scopus subject areas

  • Surgery

Cite this

Modular laser-based endoluminal ablation of the gastrointestinal tract : in vivo dose–effect evaluation and predictive numerical model. / Quero, Giuseppe; Saccomandi, Paola; Kwak, Jung-Myun; Dallemagne, Bernard; Costamagna, Guido; Marescaux, Jacques; Mutter, Didier; Diana, Michele.

In: Surgical Endoscopy, 01.01.2018.

Research output: Contribution to journalArticle

Quero, Giuseppe ; Saccomandi, Paola ; Kwak, Jung-Myun ; Dallemagne, Bernard ; Costamagna, Guido ; Marescaux, Jacques ; Mutter, Didier ; Diana, Michele. / Modular laser-based endoluminal ablation of the gastrointestinal tract : in vivo dose–effect evaluation and predictive numerical model. In: Surgical Endoscopy. 2018.
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AU - Quero, Giuseppe

AU - Saccomandi, Paola

AU - Kwak, Jung-Myun

AU - Dallemagne, Bernard

AU - Costamagna, Guido

AU - Marescaux, Jacques

AU - Mutter, Didier

AU - Diana, Michele

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background: Endoscopic submucosal dissection allows for “en bloc” removal of early gastrointestinal neoplasms. However, it is technically demanding and time-consuming. Alternatives could rely on energy-based techniques. We aimed to evaluate a predictive numerical model of thermal damage to preoperatively define optimal laser settings allowing for a controlled ablation down to the submucosa, and the ability of confocal endomicroscopy to provide damage information. Materials and methods: A Nd:YAG laser was applied onto the gastric mucosa of 21 Wistar rats on 10 spots (total 210). Power settings ranging from 0.5 to 2.5W were applied during 1–12 s, with a consequent energy delivery varying from 0.5 to 30 J. Out of the 210 samples, a total of 1050 hematoxilin–eosin stained slides were obtained. To evaluate thermal injury, the ratio between the damage depth (DD) over the mucosa and the submucosa thickness (T) was calculated. Effective and safe ablation was considered for a DD/T ratio ≤ 1 (only mucosal and submucosal damage). Confocal endomicroscopy was performed before and after ablation. A numerical model, using human physical properties, was developed to predict thermal damage. Results: No full-thickness perforations were detected. On histology, the DD/T ratio at 0.5 J was 0.57 ± 0.21, significantly lower when compared to energies ranging from 15 J (a DD/T ratio = 1.2 ± 0.3; p < 0.001) until 30 J (a DD/T ratio = 1.33 ± 0.31; p < 0.001). Safe mucosal and submucosal ablations were achieved applying energy between 4 and 12 J, never impairing the muscularis propria. Confocal endomicroscopy showed a distorted gland architecture. The predicted damage depth demonstrated a significant positive linear correlation with the experimental data (Pearson’s r 0.85; 95% CI 0.66–0.94). Conclusions: Low-power settings achieved effective and safe mucosal and submucosal ablation. The numerical model allowed for an accurate prediction of the ablated layers. Confocal endomicroscopy provided real-time thermal damage visualization. Further studies on larger animal models are required.

AB - Background: Endoscopic submucosal dissection allows for “en bloc” removal of early gastrointestinal neoplasms. However, it is technically demanding and time-consuming. Alternatives could rely on energy-based techniques. We aimed to evaluate a predictive numerical model of thermal damage to preoperatively define optimal laser settings allowing for a controlled ablation down to the submucosa, and the ability of confocal endomicroscopy to provide damage information. Materials and methods: A Nd:YAG laser was applied onto the gastric mucosa of 21 Wistar rats on 10 spots (total 210). Power settings ranging from 0.5 to 2.5W were applied during 1–12 s, with a consequent energy delivery varying from 0.5 to 30 J. Out of the 210 samples, a total of 1050 hematoxilin–eosin stained slides were obtained. To evaluate thermal injury, the ratio between the damage depth (DD) over the mucosa and the submucosa thickness (T) was calculated. Effective and safe ablation was considered for a DD/T ratio ≤ 1 (only mucosal and submucosal damage). Confocal endomicroscopy was performed before and after ablation. A numerical model, using human physical properties, was developed to predict thermal damage. Results: No full-thickness perforations were detected. On histology, the DD/T ratio at 0.5 J was 0.57 ± 0.21, significantly lower when compared to energies ranging from 15 J (a DD/T ratio = 1.2 ± 0.3; p < 0.001) until 30 J (a DD/T ratio = 1.33 ± 0.31; p < 0.001). Safe mucosal and submucosal ablations were achieved applying energy between 4 and 12 J, never impairing the muscularis propria. Confocal endomicroscopy showed a distorted gland architecture. The predicted damage depth demonstrated a significant positive linear correlation with the experimental data (Pearson’s r 0.85; 95% CI 0.66–0.94). Conclusions: Low-power settings achieved effective and safe mucosal and submucosal ablation. The numerical model allowed for an accurate prediction of the ablated layers. Confocal endomicroscopy provided real-time thermal damage visualization. Further studies on larger animal models are required.

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