Modulating cellular balance of Rps3 mono-ubiquitination by both Hel2 E3 ligase and Ubp3 deubiquitinase regulates protein quality control

Youjin Jung, Hag Dong Kim, Hee Woong Yang, Hye Jin Kim, Chang Young Jang, Joon Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

When a ribosome complex is stalled during the translation elongation process in eukaryotes, the mono-ubiquitination of Rps3 has recently been shown to be critical to ribosome quality control. We have discovered that the regulatory role of Rps3 mono-ubiquitination is controlled by a deubiquitinase. We also showed that an autophagic signal appears to be coupled to the mono-ubiquitination of Rps3p through the entrance of Ubp3p into the autophagosome in yeasts. The mono-ubiquitination of the Rps3 protein is tightly modulated by reciprocal action between the Hel2p E3 ligase and the Ubp3p deubiquitinase in yeasts and the reciprocal action between the RNF123 E3 ligase and the USP10 deubiquitinase in mammalian cells. We also found that the Ubp3p/USP10 deubiquitinases critically modulate Hel2p/RNF123-mediated Rps3p mono-ubiquitination. In addition, we found that Hel2p/RNF123 and Ubp3p/USP10 appeared to be differently localized in the ribosome complex after ultraviolet irradiation. Together, our results support a model in which coordinated ubiquitination and deubiquitination activities can finely balance the level of regulatory Rps3p mono-ubiquitination in ribosome-associated quality control and autophagy processes.

Original languageEnglish
Article numbere390
JournalExperimental and Molecular Medicine
Volume49
Issue number11
DOIs
Publication statusPublished - 2017 Nov 3

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Ubiquitin-Protein Ligases
Ubiquitination
Quality Control
Yeast
Quality control
Ribosomes
Elongation
Proteins
Cells
Irradiation
Yeasts
Autophagy
Deubiquitinating Enzymes
Eukaryota

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Modulating cellular balance of Rps3 mono-ubiquitination by both Hel2 E3 ligase and Ubp3 deubiquitinase regulates protein quality control. / Jung, Youjin; Kim, Hag Dong; Yang, Hee Woong; Kim, Hye Jin; Jang, Chang Young; Kim, Joon.

In: Experimental and Molecular Medicine, Vol. 49, No. 11, e390, 03.11.2017.

Research output: Contribution to journalArticle

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