Modulating the GSH/Trx selectivity of a fluorogenic disulfide-based thiol sensor to reveal diminished GSH levels under ER stress

Youngjin Wi, Hoa Thi Le, Peter Verwilst, Kyoung Sunwoo, Seo Jin Kim, Jung Eun Song, Hey Young Yoon, Geon Han, Jong Seung Kim, Chulhun Kang, Tae Woo Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We synthesized a fluorogenic disulfide-based naphthalimide thiol probe (ER-Naph) with a hydrophilic endoplasmic reticulum (ER)-guiding glibenclamide unit. Its ER targeting ability and high selectivity to GSH over thioredoxin, a potent competitor, were clearly demonstrated, both in solution and in vitro. Finally, a confocal microscopic investigation revealed that GSH levels in the ER were dramatically decreased under thapsigargin, brefeldin A, and tunicamycin-induced ER stress models.

Original languageEnglish
Pages (from-to)8897-8900
Number of pages4
JournalChemical Communications
Volume54
Issue number64
DOIs
Publication statusPublished - 2018 Jan 1

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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    Wi, Y., Le, H. T., Verwilst, P., Sunwoo, K., Kim, S. J., Song, J. E., Yoon, H. Y., Han, G., Kim, J. S., Kang, C., & Kim, T. W. (2018). Modulating the GSH/Trx selectivity of a fluorogenic disulfide-based thiol sensor to reveal diminished GSH levels under ER stress. Chemical Communications, 54(64), 8897-8900. https://doi.org/10.1039/c8cc04846k