Modulation of adhesion and growth of cardiac myocytes by surface nanotopography

Deok Ho Kim, Pilnam Kim, Kaph Y. Suh, Seung Kyu Choi, Sang Ho Lee, Byungkyu Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

22 Citations (Scopus)

Abstract

We have introduced well-defined nanopillar arrays of polyethylene glycol (PEG) as a platform for studying the adhesion and growth of cultured cardiomyocytes. The nanopillar arrays were fabricated by using a simple molding technique involving the placement of a patterned polyurethane acrylate mold on top of a drop-dispensed ultraviolet (UV) curable PEG polymer followed by UV exposure and mold removal. The adhesion and growth of cardiomyocytes turned out be guided by an external nanotopography, which has been characterized in terms of cell morphology and cytoskeletal arrangement. In particular, the nanopillars provided guiding posts to both elongating filopodia and expanding lamellipodia. Interestingly, the 3D growth of cardiomyocytes was mediated by the increased hydrophobicity of the nanostructured PEG substrate, indicating that the cell adhesion and growth is very sensitive to the nanotopography. The precise nanostructures of PEG-based polymer with controlled geometrical features presented in this study not only open opportunities for understanding and tailoring cell adhesion and growth, but could serve as a template for better tissue engineering by controlling cellular activities at the molecular level.

Original languageEnglish
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages4091-4094
Number of pages4
Volume7 VOLS
Publication statusPublished - 2005 Dec 1
Event2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005 - Shanghai, China
Duration: 2005 Sep 12005 Sep 4

Other

Other2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
CountryChina
CityShanghai
Period05/9/105/9/4

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ASJC Scopus subject areas

  • Bioengineering

Cite this

Kim, D. H., Kim, P., Suh, K. Y., Choi, S. K., Lee, S. H., & Kim, B. (2005). Modulation of adhesion and growth of cardiac myocytes by surface nanotopography. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 7 VOLS, pp. 4091-4094). [1615362]