Modulation of p53 expression and its role in the conversion to a fully immortalized chicken embryo fibroblast line

Shelly A. Christman, Byung W. Kong, Megan M. Landry, Hyunggee Kim, Douglas N. Foster

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have established a spontaneously immortalized chicken embryo fibroblast (CEF) cell line (SC-1) that has been in continuous culture for more than three years. This is only the second report of a spontaneously immortalized reverse transcriptase (RT)-negative chicken cell line. The SC-1 cells emerged from crisis (at about passage 29-31) with a slower growth rate than primary cells. Passage 50 SC-1 cells expressed similar levels of p53 mRNA, but slightly lower levels of p53 protein than passage 6 CEF cells. By passage 120, p53 mRNA levels were significantly decreased in the SC-1 cells, while protein levels were slightly increased compared to passage 6 CEF cells. However, functional analysis of p53 revealed reduced activity in later passage SC-1 cells. Other p53-related genes including p21WAF1, p27Kip1, MDM-2, and the p16 INK4a alternate reading frame (ARF) sequence showed similar patterns of differential mRNA expression. Levels of p15INK4b mRNA and protein were dramatically decreased in SC-1 cells, suggesting that the Rb pathway also has been compromised. Telomerase expression was undetectable in SC-1 cells. Fluorescence-activated cell sorting analysis showed that SC-1 and primary cells contained a similar proportion of G0/G1 phase cells, unlike the only other spontaneously immortalized chicken cell line (DF-1). The present study suggests that alterations in the p53 and Rb pathways cause fluctuations in expression levels of important cell-cycle regulatory genes during crucial transition periods as the SC-1 spontaneously immortalized chicken fibroblast cells progress toward becoming a fully committed cell line.

Original languageEnglish
Pages (from-to)6705-6715
Number of pages11
JournalFEBS Letters
Volume579
Issue number30
DOIs
Publication statusPublished - 2005 Dec 19

Fingerprint

Fibroblasts
Chickens
Embryonic Structures
Cells
Modulation
Messenger RNA
Genes
Cell Line
Functional analysis
Proteins
Telomerase
RNA-Directed DNA Polymerase
Cyclin-Dependent Kinase Inhibitor p15
Sorting
Fluorescence
cdc Genes
Reading Frames
Cell Cycle Resting Phase
p53 Genes
G1 Phase

Keywords

  • Chicken embryo fibroblast cell line
  • p15
  • p53
  • Spontaneous immortalization

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Modulation of p53 expression and its role in the conversion to a fully immortalized chicken embryo fibroblast line. / Christman, Shelly A.; Kong, Byung W.; Landry, Megan M.; Kim, Hyunggee; Foster, Douglas N.

In: FEBS Letters, Vol. 579, No. 30, 19.12.2005, p. 6705-6715.

Research output: Contribution to journalArticle

Christman, Shelly A. ; Kong, Byung W. ; Landry, Megan M. ; Kim, Hyunggee ; Foster, Douglas N. / Modulation of p53 expression and its role in the conversion to a fully immortalized chicken embryo fibroblast line. In: FEBS Letters. 2005 ; Vol. 579, No. 30. pp. 6705-6715.
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