Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle

Narae Kim, Yunho Jang, Jin Kyoung Kim, Soyoon Ryoo, Ka Hee Kwon, Miso Kim, Shin Seok Kang, Hyeon Seop Byeon, Hee Soo Lee, Young Hee Lim, Jae Myung Kim

Research output: Contribution to journalArticle

Abstract

The aim of this study was to investigate the molecular characteristics and to conduct a comparative genomic analysis of Mycobacterium (M.) bovis strain 1595 isolated from a native Korean cow. Molecular typing showed that M. bovis 1595 has spoligotype SB0140 with mycobacterial interspersed repetitive units-variable number of tandem repeats typing of 4-2-5-3-2-7-5-5-4-3-4-3-4-3, representing the most common type of M. bovis in Korea. The complete genome sequence of strain 1595 was determined by single-molecule real-time technology, which showed a genome of 4351712 bp in size with a 65.64% G + C content and 4358 protein-coding genes. Comparative genomic analysis with the genomes of Mycobacterium tuberculosis complex strains revealed that all genomes are similar in size and G + C content. Phylogenetic analysis revealed all strains were within a 0.1% average nucleotide identity value, and MUMmer analysis illustrated that all genomes showed positive collinearity with strain 1595. A sequence comparison based on BLASTP analysis showed that M. bovis AF2122/97 was the strain with the greatest number of completely matched proteins to M. bovis 1595. This genome sequence analysis will serve as a valuable reference for improving understanding of the virulence and epidemiologic traits among M. bovis isolates in Korea.

Original languageEnglish
Pages (from-to)333-341
Number of pages9
JournalJournal of Veterinary Science
Volume18
DOIs
Publication statusPublished - 2017

Fingerprint

Mycobacterium bovis
Genome
genomics
genome
cattle
Base Composition
Korea
Korean Peninsula
Mycobacterium tuberculosis complex
Molecular Typing
Minisatellite Repeats
minisatellite repeats
Mycobacterium
Mycobacterium tuberculosis
Sequence Analysis
Virulence
Proteins
virulence
Nucleotides
sequence analysis

Keywords

  • Cattle
  • Genomics
  • Korea
  • Mycobacterium bovis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle. / Kim, Narae; Jang, Yunho; Kim, Jin Kyoung; Ryoo, Soyoon; Kwon, Ka Hee; Kim, Miso; Kang, Shin Seok; Byeon, Hyeon Seop; Lee, Hee Soo; Lim, Young Hee; Kim, Jae Myung.

In: Journal of Veterinary Science, Vol. 18, 2017, p. 333-341.

Research output: Contribution to journalArticle

Kim, N, Jang, Y, Kim, JK, Ryoo, S, Kwon, KH, Kim, M, Kang, SS, Byeon, HS, Lee, HS, Lim, YH & Kim, JM 2017, 'Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle', Journal of Veterinary Science, vol. 18, pp. 333-341. https://doi.org/10.4142/jvs.2017.18.S1.333
Kim, Narae ; Jang, Yunho ; Kim, Jin Kyoung ; Ryoo, Soyoon ; Kwon, Ka Hee ; Kim, Miso ; Kang, Shin Seok ; Byeon, Hyeon Seop ; Lee, Hee Soo ; Lim, Young Hee ; Kim, Jae Myung. / Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle. In: Journal of Veterinary Science. 2017 ; Vol. 18. pp. 333-341.
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