Molecular and serologic survey of Orientia tsutsugamushi infection among field rodents in southern Cholla Province, Korea

Hyun J. Song, Seung Yong Seong, Myung S. Huh, Sae Gwang Park, Won Jong Jang, Sun-Ho Kee, Kai Hwan Kim, Sun Chun Kim, Myung S. Choi, Ik Sang Kim, Woo Hyun Chang

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Abstract

Field rodents were collected from six areas in southern Cholla Province, Korea from October to December 1993. Twenty-eight (24%) of the 119 Apodemus agrarius were seropositive (> 1:10) for Orientia tsutsugamushi by the passive hemagglutination assay (PHA). Of the seropositive cases, 11 specimens had antibody titers greater than 1:80. No seropositive specimens were found among the eight Crocidura lasiura collected. On the other hand, the polymerase chain reaction (PCR) amplified about 520 basepairs of a gene encoding the 56- kD protein from the genomic DNA of 12 strains of O. tsutsugamushi tested. This target DNA sequence was amplified from the 11 (8.7%) blood specimens of A. agrarius, and one of the eight C. lasiura also showed evidence of O. tsutsugamushi infection by PCR. Only one of the PCR-positive samples was also PHA-positive. These results suggest that the PCR combined with a serologic assay more accurately detects the degree of infection of rodents with rickettsiae-causing scrub typhus in epidemiologic surveys.

Original languageEnglish
Pages (from-to)513-518
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume58
Issue number4
Publication statusPublished - 1998 May 5
Externally publishedYes

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Orientia tsutsugamushi
Korea
Rodentia
Polymerase Chain Reaction
Hemagglutination
Infection
Scrub Typhus
Murinae
Rickettsia
Surveys and Questionnaires
Antibodies
DNA
Genes
Proteins

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Molecular and serologic survey of Orientia tsutsugamushi infection among field rodents in southern Cholla Province, Korea. / Song, Hyun J.; Seong, Seung Yong; Huh, Myung S.; Park, Sae Gwang; Jang, Won Jong; Kee, Sun-Ho; Kim, Kai Hwan; Kim, Sun Chun; Choi, Myung S.; Kim, Ik Sang; Chang, Woo Hyun.

In: American Journal of Tropical Medicine and Hygiene, Vol. 58, No. 4, 05.05.1998, p. 513-518.

Research output: Contribution to journalArticle

Song, HJ, Seong, SY, Huh, MS, Park, SG, Jang, WJ, Kee, S-H, Kim, KH, Kim, SC, Choi, MS, Kim, IS & Chang, WH 1998, 'Molecular and serologic survey of Orientia tsutsugamushi infection among field rodents in southern Cholla Province, Korea', American Journal of Tropical Medicine and Hygiene, vol. 58, no. 4, pp. 513-518.
Song, Hyun J. ; Seong, Seung Yong ; Huh, Myung S. ; Park, Sae Gwang ; Jang, Won Jong ; Kee, Sun-Ho ; Kim, Kai Hwan ; Kim, Sun Chun ; Choi, Myung S. ; Kim, Ik Sang ; Chang, Woo Hyun. / Molecular and serologic survey of Orientia tsutsugamushi infection among field rodents in southern Cholla Province, Korea. In: American Journal of Tropical Medicine and Hygiene. 1998 ; Vol. 58, No. 4. pp. 513-518.
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