Molecular, cellular, and developmental biology: Gene pathways and cell cycle-related genes in cultured avian primordial germ cells

D. Rengaraj, B. R. Lee, J. W. Choi, S. I. Lee, H. W. Seo, T. H. Kim, H. J. Choi, Gwonhwa Song, J. Y. Han

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Primordial germ cells (PGC) from early embryos are applicable to various kinds of research, including the production of transgenic animals. Primordial germ cells eventually migrate and differentiate into germ cells in the gonads, where they settle and rapidly proliferate. However, the proliferation rate of PGC is low in early embryos, and there are many significant pathways that mediate PGC activity. Therefore, in vitro culture of PGC from early embryos with efficient growth factors has been necessary. Recently, we cultured chicken PGC from embryonic d 2.5 with basic fibroblast growth factor and characterized the PGC through analysis of cell morphology, survival, proliferation, and apoptosis. However, large-scale analyses of genes expressed in cultured PGC and the genes involved in associated pathways are limited. The objective of the present investigation was to identify the signaling and metabolic pathways of expressed genes by microarray comparison between PGC and their somatic counterpart, chicken embryonic fibroblasts (CEF). We identified 795 genes that were expressed more predominantly in PGC and 824 genes that were expressed more predominantly in CEF. Among the predominant genes in PGC, 201 were differentially identified in 106 pathways. Among the predominant genes in CEF, 242 were differentially identified in 99 pathways. To further validate the genes involved in at least one candidate pathway, those involved in the cell cycle (12 predominant genes in PGC and 8 predominant genes in CEF) were examined by real-time PCR. To the best of our knowledge, this study is the first to investigate signaling and metabolic pathways in cultured PGC.

Original languageEnglish
Pages (from-to)3167-3177
Number of pages11
JournalPoultry Science
Volume91
Issue number12
DOIs
Publication statusPublished - 2012 Dec 1
Externally publishedYes

Fingerprint

Developmental Genes
cdc Genes
Developmental Biology
Germ Cells
Cell Biology
Molecular Biology
cell cycle
germ cells
Biological Sciences
genes
Genes
Chickens
fibroblasts
chickens
Fibroblasts
Embryonic Structures
embryo (animal)
Metabolic Networks and Pathways
cultured cells
biochemical pathways

Keywords

  • Chicken
  • Chicken embryonic fibroblast
  • Gene pathway
  • Microarray
  • Primordial germ cell

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Molecular, cellular, and developmental biology : Gene pathways and cell cycle-related genes in cultured avian primordial germ cells. / Rengaraj, D.; Lee, B. R.; Choi, J. W.; Lee, S. I.; Seo, H. W.; Kim, T. H.; Choi, H. J.; Song, Gwonhwa; Han, J. Y.

In: Poultry Science, Vol. 91, No. 12, 01.12.2012, p. 3167-3177.

Research output: Contribution to journalArticle

Rengaraj, D. ; Lee, B. R. ; Choi, J. W. ; Lee, S. I. ; Seo, H. W. ; Kim, T. H. ; Choi, H. J. ; Song, Gwonhwa ; Han, J. Y. / Molecular, cellular, and developmental biology : Gene pathways and cell cycle-related genes in cultured avian primordial germ cells. In: Poultry Science. 2012 ; Vol. 91, No. 12. pp. 3167-3177.
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