Molecular characterisation of the Cu/Zn superoxide dismutase gene (ZjSOD1) induced by salt stress in Zoysia japonica

Seung Ho Jung, Min Jeong Hong, Dae Yeon Kim, Jae Yoon Kim, Je Hyeong Jung, Yong Weon Seo

Research output: Contribution to journalArticle

Abstract

NaCl was used to evaluate the molecular response to salt stress in Zoysia japonica. A 452-bp cDNA clone, which showed high similarity to other plant Cu/Zn superoxide dismutase (SOD) gene sequences, was selected and designated Zoysia japonica Cu/Zn superoxide dismutase 1 (ZjSOD1). Southern blot analysis showed the presence of at least two copies of the ZjSOD1 gene in Z. japonica. ZjSOD1 transcripts increased with exposure to a high salt concentration (300 mM NaCl). ZjSOD1 transcript levels increased in non-immersed tissues such as stolons and their tips, as well as in stolon tissue that had been immersed in 300 mM NaCl for 36 h. The highest level of ZjSOD1 gene expression was observed in stolon tips. However, ZjSOD1 transcript levels were higher in immersed stolons than in non-immersed stolons, or their tips, during the salt stress treatment. Molecular characterisation of the ZjSOD1 gene may provide clues about the response mechanism to salt stress in the stolons of Z. japonica and other turfgrasses.

Original languageEnglish
Pages (from-to)640-646
Number of pages7
JournalJournal of Horticultural Science and Biotechnology
Volume87
Issue number6
Publication statusPublished - 2012 Nov 1

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Zoysia japonica
salt stress
superoxide dismutase
Salts
stolons
Genes
genes
Superoxide Dismutase-1
turf grasses
salt concentration
Southern blotting

ASJC Scopus subject areas

  • Horticulture
  • Genetics

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Molecular characterisation of the Cu/Zn superoxide dismutase gene (ZjSOD1) induced by salt stress in Zoysia japonica. / Jung, Seung Ho; Hong, Min Jeong; Kim, Dae Yeon; Kim, Jae Yoon; Jung, Je Hyeong; Seo, Yong Weon.

In: Journal of Horticultural Science and Biotechnology, Vol. 87, No. 6, 01.11.2012, p. 640-646.

Research output: Contribution to journalArticle

Jung, Seung Ho ; Hong, Min Jeong ; Kim, Dae Yeon ; Kim, Jae Yoon ; Jung, Je Hyeong ; Seo, Yong Weon. / Molecular characterisation of the Cu/Zn superoxide dismutase gene (ZjSOD1) induced by salt stress in Zoysia japonica. In: Journal of Horticultural Science and Biotechnology. 2012 ; Vol. 87, No. 6. pp. 640-646.
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