Molecular characterization and bitter taste formation of tryptic hydrolysis of 11S glycinin

Mi Ryung Kim, Sang-Yun Choi, Cherl Ho Lee

Research output: Contribution to journalArticle

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Abstract

The molecular size reduction and the formation of bitterness during a tryptic hydrolysis of soybean 11S glycinin were determined by using quantitative analysis and organoleptic evaluation. The 11S glycinin of 90% purity was prepared by cryoprecipitation and Con A Sepharose 4B affinity chromatography, and hydrolyzed with trypsin in a pH-stat reactor for 4h. Bitterness was formed within 1 h of hydrolysis, and then slowly increased up to 3.5x10-5M quinine-HCl equivalent. The extent of hydrolysis (DH) was 7% at 1 h and increased up to 12% by the end of the reaction. The α-amino nitrogen content increased from an initial 0.7 mM to 7 mM at the end of the period. The SDS-PAGE analysis showed that the acidic subunit of 11S glycinin was mostly hydrolyzed. The GP-HPLC analysis indicated that the bitterness was mainly contributed by the peptide fractions of molecular weights of 360- 2,100 Da.

Original languageEnglish
Pages (from-to)509-513
Number of pages5
JournalJournal of Microbiology and Biotechnology
Volume9
Issue number4
Publication statusPublished - 1999 Aug 1

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Hydrolysis
Affinity chromatography
Quinine
Affinity Chromatography
Soybeans
Sepharose
Trypsin
Peptides
Polyacrylamide Gel Electrophoresis
Nitrogen
Molecular Weight
Molecular weight
High Pressure Liquid Chromatography
Chemical analysis
glycinin
concanavalin A-sepharose

Keywords

  • Bitterness
  • Enzymatic hydrolysis
  • Peptides
  • Soybean protein

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Molecular characterization and bitter taste formation of tryptic hydrolysis of 11S glycinin. / Kim, Mi Ryung; Choi, Sang-Yun; Lee, Cherl Ho.

In: Journal of Microbiology and Biotechnology, Vol. 9, No. 4, 01.08.1999, p. 509-513.

Research output: Contribution to journalArticle

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