Molecular characterization of a cDNA encoding putative calcium binding protein, HvCaBP1, induced during kernel development in barley (Hordeum vulgare L.)

C. S. Jang, M. S. Lee, J. Y. Kim, D. S. Kim, Yong Weon Seo

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Seed development is known to involve complex physiological and molecular events. In order to gain information on the molecular events that occur in the grains of barley during kernel development, we conducted suppression subtractive hybridization (SSH) using grains of barley cv. Karl at 14 days after fertilization (DAF) as the tester and grains at 5 DAF as the driver. We isolated an SSH clone that showed homology with a specific calcium binding protein in rice called EFA27. Screening the cDNA library, we identified two clones as a calcium binding protein. These clones, each carrying one calcium-binding EF-hand motif, were designated HvCaBP1 (Hordeum vulgare Calcium Binding Protein 1). HvCaBP1 possesses an N-terminal region with a conserved single Ca2+-binding EF-hand motif and one transmembrane helix. Northern hybridization showed that the highest expression occurred in grains and that expression increased in kernels at 8 DAF. As shown in situ hybridization, the HvCaBP1 gene was highly expressed in the embryo and tissues of the endosperm near the embryo and was detected in the vascular tissues of the glume in the kernel at 8 DAF. Accumulation of HvCaBP1 mRNAs subsequently increased in vegetative tissues for up to 48 h after abscisic acid (ABA) treatment. Transcripts of HvCaBP1 mRNAs may be regulated by endogenous ABA in the grains during kernel development.

Original languageEnglish
Pages (from-to)64-70
Number of pages7
JournalPlant Cell Reports
Volume22
Issue number1
DOIs
Publication statusPublished - 2003 Aug 1

Fingerprint

calcium-binding proteins
seed development
Hordeum vulgare
barley
EF hand motifs
suppression subtractive hybridization
clones
embryo (plant)
abscisic acid
calcium
vascular tissues
seeds
acid treatment
cDNA libraries
Northern blotting
in situ hybridization
endosperm
screening
rice

Keywords

  • Abscisic acid
  • Barley
  • Calcium binding protein
  • Kernel development

ASJC Scopus subject areas

  • Plant Science

Cite this

Molecular characterization of a cDNA encoding putative calcium binding protein, HvCaBP1, induced during kernel development in barley (Hordeum vulgare L.). / Jang, C. S.; Lee, M. S.; Kim, J. Y.; Kim, D. S.; Seo, Yong Weon.

In: Plant Cell Reports, Vol. 22, No. 1, 01.08.2003, p. 64-70.

Research output: Contribution to journalArticle

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