Molecular characterization of an S-adenosylmethionine decarboxylase gene from floral organ differentiating axillary bud in calamondin (X Citrofortunella mitis)

S. K. Kang, C. S. Jang, H. Y. Kim, S. H. Yun, H. J. An, J. H. Park, J. W. Haam, Yong Weon Seo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Calamondin (X Citrofortunella mitis J. Ingram & H. E. Moore) is known for its ability to develop floral organs to flower in the adult phase during the four seasons. To provide information on molecular events during floral differentiation from axillary buds, we constructed a cDNA library from floral differentiating axillary buds of adult phase calamondin. Ninety-six cDNA clones were randomly selected from this library and sequenced. Ninety-six non-redundant ESTs were identified. Two clones encoded S-adenosylmethione decarboxylase, a key enzymes in polyamine biosynthesis. CmSAMDC 1 (Citrofortunella mitis s-adenosylmethionine decarboxylase 1) contained an 1083 bp ORF that encoded a putative SAMDC precursor of 361 amino acids. Northern blot analysis revealed that CmSAMDC 1 was expressed in axillary buds prior to floral differentiation and in axillary buds immediately after floral differentiation, in immature flower (5 mm), in fully developed flowers (120 mm long), in floral parts (petals, pistils, and stamens) of trees in the adult phase of development, but not in leaves or axillary buds of juvenile phase nucellar seedlings or leaf tissue from trees in adult phase of development. In situ hybridization revealed expression of the CmSAMDC 1 gene in the floral apex of axillary buds after differentiation, and in the phloem of axillary buds and petioles. These results suggest that SAMDC could play a role in actively differentiating and developing reproductive and vegetative organs.

Original languageEnglish
Pages (from-to)742-747
Number of pages6
JournalJournal of Horticultural Science and Biotechnology
Volume78
Issue number5
Publication statusPublished - 2003 Sep 1

Fingerprint

Citrus madurensis
Adenosylmethionine Decarboxylase
adenosylmethionine decarboxylase
buds
Genes
genes
Clone Cells
Phloem
Carboxy-Lyases
flowers
Expressed Sequence Tags
Polyamines
Gene Library
Seedlings
Northern Blotting
Open Reading Frames
Libraries
In Situ Hybridization
clones
Complementary DNA

ASJC Scopus subject areas

  • Plant Science
  • Applied Microbiology and Biotechnology
  • Horticulture
  • Biotechnology

Cite this

Molecular characterization of an S-adenosylmethionine decarboxylase gene from floral organ differentiating axillary bud in calamondin (X Citrofortunella mitis). / Kang, S. K.; Jang, C. S.; Kim, H. Y.; Yun, S. H.; An, H. J.; Park, J. H.; Haam, J. W.; Seo, Yong Weon.

In: Journal of Horticultural Science and Biotechnology, Vol. 78, No. 5, 01.09.2003, p. 742-747.

Research output: Contribution to journalArticle

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