Molecular characterization of pepper germin-like protein as the novel PR-16 family of pathogenesis-related proteins isolated during the resistance response to viral and bacterial infection

Chang Jin Park, Jong Min An, Yun Chul Shin, Ki Jeong Kim, Boo Ja Lee, Kyung Hee Paek

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

To understand the molecular defense mechanism controlling the hypersensitive response (HR) better, we examined the hot pepper plant (Capsicum annuum L. cv. Bugang), which exhibits an HR in response to infection by Tobacco mosaic virus pathotype P0 (TMV-P0). A full-length cDNA clone was isolated by differential screening of a cDNA library that was constructed with mRNA extracted from hot pepper leaves during the resistance response to TMV-P0. The predicted amino acid sequence of the cDNA clone exhibited a high sequence similarity to germin-like protein (GLP). The CaGLP1 (Capsicum annuum GLP1) cDNA contains a single open reading frame of 660 bp encoding 220 amino acid residues. Upon inoculation with TMV or Xanthomonas, CaGLP1 transcripts were specifically accumulated in the incompatible interaction but not in the compatible interaction. In plants treated with salicylic acid (SA) or ethephon, which are signal molecules in the defense-related signal transduction pathway, CaGLP1 transcripts were accumulated rapidly. As far as we know, this is the first report that plant GLPs can be specifically induced during a defense response against viral infection. These data suggest that in the hot pepper plant CaGLP1 may be involved in the defense response to viral pathogens, and thus be classified as a new family of PR proteins, 'PR-16'.

Original languageEnglish
Pages (from-to)797-806
Number of pages10
JournalPlanta
Volume219
Issue number5
DOIs
Publication statusPublished - 2004 Sep 1

Fingerprint

Capsicum
pathogenesis-related proteins
sweet peppers
Virus Diseases
Capsicum annuum
bacterial infections
Bacterial Infections
hot peppers
Tobacco mosaic virus
pathotypes
hypersensitive response
Proteins
proteins
Tobacco Mosaic Virus
Complementary DNA
clones
Xanthomonas
new family
ethephon
defense mechanisms

Keywords

  • Capsicum
  • Differential screening
  • Germin-like protein
  • Hypersensitive response
  • Tobacco mosaic virus
  • Xanthomonas

ASJC Scopus subject areas

  • Plant Science

Cite this

Molecular characterization of pepper germin-like protein as the novel PR-16 family of pathogenesis-related proteins isolated during the resistance response to viral and bacterial infection. / Park, Chang Jin; An, Jong Min; Shin, Yun Chul; Kim, Ki Jeong; Lee, Boo Ja; Paek, Kyung Hee.

In: Planta, Vol. 219, No. 5, 01.09.2004, p. 797-806.

Research output: Contribution to journalArticle

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