Molecular characterization of plasmids encoding CTX-M β-Lactamases and their associated addiction systems circulating among escherichia coli from retail chickens, chicken farms, and slaughterhouses in Korea

Su Jin Jo, Gun-Jo Woo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Extended-spectrum β-lactamases (ESBLs), particularly those of the CTX-M types, are the predominant resistance determinants of Escherichia coli that are rapidly spreading worldwide. To determine CTX-M types, E. coli isolates were collected from retail chickens (n = 390) and environmental samples from chicken farms (n = 32) and slaughterhouses (n = 67) in Korea. Fifteen strains harboring blaCTX-M genes were isolated from 358 E. coli isolates. The most common CTX-M type was eight of CTX-M-15, followed by six of CTX-M-1 and one of CTX-M- 14. The blaCTX-M genes were identified in the isolates from retail chickens (n = 9), followed by feces, water pipes, floors, and walls. Conjugations confirmed the transferability of the plasmids carrying blaCTX-M genes to the recipient E. coli J53 strain. Furthermore, eight addiction systems carried by the replicons in CTX-M types were confirmed. The dominant system was identified as ccdAB, vagCD, and pndAC in donor strains and transconjugants. The clonal relationship between the two strains carrying blaCTX-M genes indicates that E. coli may transmit from the farm to retail chickens, suggesting a possible public health risk. Our findings demonstrate that the detection of CTX-M types in E. coli isolates is important for tracking ESBL production in animals, and suggest linkage of multiple addiction systems in plasmids bearing blaCTX-M genes.

Original languageEnglish
Pages (from-to)270-276
Number of pages7
JournalJournal of Microbiology and Biotechnology
Volume26
Issue number2
DOIs
Publication statusPublished - 2015 Nov 12

Keywords

  • Addiction systems
  • Chicken farms
  • CTX-M types
  • Escherichia coli
  • Retail chickens
  • Slaughterhouses

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Molecular characterization of plasmids encoding CTX-M β-Lactamases and their associated addiction systems circulating among escherichia coli from retail chickens, chicken farms, and slaughterhouses in Korea'. Together they form a unique fingerprint.

  • Cite this